摘要
目的评价分析脂多糖在创伤性颅脑损伤(TBI)炎症反应中的应用价值。方法选择2014年6月~2016年12月期间佛山市南海区第三人民医院脑科和南方医科大学珠江医院神经外科收治的TBI患者86例,并按照格拉斯哥昏迷评分分为3组:轻度组33例、中度组24例、重度组29例;并且选取同期来本院进行体检的健康者28例作为对照组。通过ELISA方法检测研究对象血清中TNF-α、IL-6、IL-1β,GREISS法检测一氧化氮含量。结果轻度组、中度组及重度组患者入院时的血清TNF-α浓度均有升高,其中第3天为最高,第5、7天已逐渐降低,但仍高于对照组。轻度组、中度组及重度组患者入院时的血清IL-6浓度均升高,其中第5天为最高,第7天已开始降低,仍高于对照组;TBI严重分级与血清IL-6浓度(r=0.925,P<0.05)。轻度组、中度组及重度组患者入院时的血清IL-1β浓度均升高,第1、3、5天缓慢增加,第7天增加更加显著,重度组升高幅度明显高于对照组、轻度组和中度组(P<0.05)。TBI严重分级与血清IL-1β浓度(r=1.267,P<0.05)。轻度组、中度组及重度组患者入院时的血清一氧化氮浓度有升高,其中第3天为升高较明显,第5、7天已逐渐降低,仍高于对照组;TBI严重分级与血清一氧化氮浓度(r=0.847,P<0.05)。结论脂多糖在TBI患者体内存在不同程度的炎症反应;血清中TNF-α、IL-6、IL-1β、一氧化氮浓度变化与TBI程度密切相关;如果浓度持续保持较高水平,这就说明脑组织损伤严重,预后效果不佳。
Objective To evaluate the value of lipopolysaccharide （LPS） in invasive brain injury （TBI） inflammatory response （Inflammation）. Methods Eighty-six patients with TBI in Third People＇s Hospital of Nanhai Foshan were enrolled.They were divided into 3 groups according to the Glasgow coma scale （GCS） score, with mild group of 33 cases, moderate group of 24 cases and severe group of 29 cases. Twenty-eight cases with health examination in the same period were selected as a control group.The levels of nitric oxide （NO） in serum were detected by GREISS. The levels of TNF-α, IL-6, IL-1β and were measured by ELISA. Results The levels of serum TNF-α were increased in the mild, moderate and severe groups, and the third day was the highest. The levels of serum TNF-α on the 5th and 7th day were gradually decreased and still higher than the control group. The levels of serum IL-6 in the mild, moderate and severe groups were higher than those in the control group （P〈0.05）. The level of IL-6 in the mild and moderate group was significantly higher than that in the control group （r=0.925, P〈0.05）. The levels of serum IL-1β in the mild group, moderate group and severe group were higher than those in the control group, and the increasement was significant on the third, fifth day （P〈0.05）. The severity of TBI was correlated with serum IL-1β concentration （r=1.267, P〈0.05）. The levels of serum NO in the mild, moderate and severe groups were significantly higher than those in the control group （P〈0.05）, and serum NO levels on the third day was higher than that in the control group. NO concentration was correlated with the severity of TBI （r=0.847, P〈0.05）. Conclusion The changes of TNF-a, IL-6, IL-1β and NO in serum are closely related to the degree of TBI. High NO concentration means a serious brain tissue injury and poor prognosis.
出处
《分子影像学杂志》
2017年第3期304-307,共4页
Journal of Molecular Imaging
基金
广东省佛山市医学类科技攻关项目(2015AB000352)
