摘要
目的:探讨分泌性靶抗原-6(ESAT-6)、肿瘤坏死因子-α(TNF-α)、白细胞介素-10(IL-10)和干扰素-γ(IFN-γ)对于早期诊断结核性脑膜炎(TBM)的临床应用价值。方法:选取48例TBM患者为研究组,29例非结核性中枢神经系统感染患者为对照组,35例普通头痛患者为正常组。采用ELISA法分别检测所有患者脑脊液中的ESAT-6、IL-10、TNF-α和IFN-γ水平。结果:研究组的ESAT-6、TNF-α、IFN-γ水平与对照组相比增高,研究组与对照组的ESAT-6、IL-10、TNF-α、IFN-γ水平均高于正常组,差异均有统计学意义(P<0.05)。研究组的ESAT-6与IFN-γ水平呈正相关(r=0.96,P<0.05)。4项指标联合检测的灵敏度明显高于使用ESAT-6、IL-10、TNF-α、IFN-γ单一指标,差异均具有统计学意义(χ2=10.936,18.104,12.647,13.978;P<0.05)。结论:ESAT-6、TNF-α、IFN-γ和IL-10在TBM患者脑脊液中的表达水平显著升高,对早期诊断TBM具有重要的临床价值,而且4项联合检测较单一检测的诊断效果更佳。
Objective： To investigate the early secreting antigenic target-6 （ESAT-6）, tumor necrosis factor-or （TNF-α）, interferon-γ （IFN- γ） and interleukin-10 （IL-10） for early diagnosis of tuberculous meningitis （TBM）. Methods： Forty-eight patients with TBM were enrolled as study group, 29 non-tuberculous central nervous system infection patients were enrolled as control group, 35 cases of normal headache patients were enrolled as normal group. The levels of ESAT-6,TNF- α, IFN- γ and IL-10 levels in CSF of all patients were detected by ELISA method. Results： The levels of ESAT-6, TNF- α, IFN- γ and IL-10 of patients in the study group were significantly higher than those in the control group. The levels of ESAT-6, IL-10, TNF-α and IFN-γ in the groups study and control group were significantly higher than those in the normal group （P〈0.05）. It had significantly positive correlation between ESAT-6 and IFN- γ of patients in the study group （r=0.96,P〈0.05）. The sensitivity of the combined detection of 4 indexes was significantly higher than that of one index （x^2=10.936, 18.104, 12.647, 13.978;P〈0.05）. Conclusion： The levels of ESAT-6, TNF- α, IFN- γ and IL-10 in cerebrospinal fluid of TBM patients were significantly increased, which has important clinical value for early diagnosis of TBM. Four joint detection is better than the single detection of the diagnosis.
作者
刘永莉
李明武
赖明红
万荣
LIU Yong-li LI Ming-wu LAI Ming-hong WANG Rong(Department of Tuberculosis, Third People＇s Hospital of Kunming, Kunming 650041, China)
出处
《神经损伤与功能重建》
2017年第4期327-329,共3页
Neural Injury and Functional Reconstruction
