摘要
目的探讨IL-12、IL-18、IL-10和TNF-α与TGF-β1在结核病免疫发病机制中的作用。方法用双抗体夹心ELISA及ABC-ELISA法检测49例结核性胸腔积液患者胸水中和血清中以上5种细胞因子的水平,并进行相关性分析。结果从总体来看,除IL-18外,IL-12、TNF-α、IL-10胸水中含量都远远超过血清中的含量,而TGF-β1血清中含量远远超过胸水中含量(P<0.05或0.01)。结论 IL-18可能用作加强疫苗保护性的一种辅助因子;某种细胞因子不是对所有患者都有效,其作用与体内细胞因子间的平衡有关。
Objective It is to approach the role of IL-10,IL-12,IL-18,TNF-α and TGF-β1 in the immunopathogenesis of tuberculosis.Methods The concentrations of IL-10,IL-12,IL-18,TNF-α and TGF-β1 in pleural fluid（PF） and serum were measured by sandwich ABC-ELISA and sandwich double antibody ELISA Kits in 49 patients with tuberculosis pleural effusion,and correlative factor analysis was performed.Results The concentrations of IL-10,IL-12 and TGF-β1 in PF were far higher than that in serum;but the level of TGF-β in serum was higher than that in PF（P0.05 or 0.01）.There was no significant difference between the levels of IL-18 in serum and PF.Conclusion IL-18 may be used as an adjuvant agent to enhance the efficacy of vaccine.The role of a certain cytokine isn＇t utility in all patients,it is related with the balance of cytokine network.
出处
《现代中西医结合杂志》
CAS
2011年第15期1831-1832,1884,共3页
Modern Journal of Integrated Traditional Chinese and Western Medicine
关键词
结核
细胞因子
免疫机制
tuberculosis
cytokine
immunopathogenesis
