摘要
目的:探索非急性感染期间,腺样体和扁桃体组织中EB病毒(EBV)、人博卡病毒(HBoV)及多瘤病毒KI和WU(KIPyV和WUPyV)检测的检出率以及4种病毒的季节性分布特征。方法:采用实时聚合酶链反应检测慢性扁桃体炎和腺体肥大儿童中EBV,HBoV,多瘤病毒KIPyV和WUPyV的DNA表达。患者分组如下:腺样体肥大组,慢性扁桃体炎组和生理性扁桃体肥大组。还研究了这些病毒的表达与年龄,性别,反复感染和气道阻塞的关系。此外,还分析了这些病毒在腺样体和扁桃体组织中的检测率的季节变化。结果:研究发现,HBoV是腺样体标本中最常见的病毒,检出率为43.1%。在慢性扁桃体炎和扁桃体肥大标本中,EBV是最常见的病毒(检出率分别为53.8%和32.0%)。虽然在腺样体肥大组的儿童中全年都能检测到HBoV和EBV阳性,但在慢性扁桃体炎的儿童中全年也均能检测到EBV阳性。结论:在无症状慢性扁桃体炎及腺样体肥大儿童样本中全年都能检测到HBoV和EBV,这表明这些病毒可能持续存在于上呼吸道的淋巴上皮组织中。病毒持久性存在可能对淋巴样肥大的发展有致病潜力,并对炎症有慢性刺激作用。
Objective：To investigate the seasonal disturbations and the rates of detection of Epstein-Barr virus（EBV）,Human Bocavirus（HBoV）,and polyomaviruses KI and WU（KIPyV and WUPyV）in adenoid and tonsil tissues during the absence of acute infection symptoms.Method：DNA expressions of EBV,HBoV,polyomaviruses KIPyV and WUPyV were investigated in children with chronic tonsillar and adenoidal diseases using real-time polymerase chain reaction.The patients were divided into three group：adenoid group,chronic tonsillit group and hypertrophic tonsillitis group.The relationships of the expressions of these viruses with age,gender,recurrent infection and airway obstruction were analyzed.Seasonal variations in rates of detection of these viruses in adenoid and tonsil tissues were also investigated.Result：Considering adenoid specimens,HBoV was found to be the most frequent virus with the rate of 43.1%.In specimens of chronic tonsillitis and hypertrophic tonsils,EBV was the most frequently encountered virus（53.8%,and 32.0%,respectively）.In children with hypertrophic adenoids,while HBoV was detected to be positive throughout the year,EBV was present throughout the year in children with recurrent tonsillitis.Conclusion：The detection of HBoV and EBV throughout the year in samples of children with asymptomatic chronic adenotonsillar diseases may indicate that these viruses may remain persistently in lymphoepithelial tissues of upper respiratory tract.Virus persistence may have a pathogenetic potential for development of lymphoid hypertrophy and a chronic stimulatory effect for inflammation.
出处
《临床耳鼻咽喉头颈外科杂志》
CAS
北大核心
2017年第14期1082-1085,1088,共5页
Journal of Clinical Otorhinolaryngology Head And Neck Surgery
