摘要
为研究五味子醇甲、五味子醇乙、五味子甲素、五味子乙素、五味子丙素、五味子酯甲和五味子酯乙这7种木脂素对草鱼肝细胞的毒性作用和安全浓度,采用草鱼原代肝细胞作为实验模型。实验组加入1~1 000μmol/L不等的待测木脂素,溶剂对照组加入等体积的二甲基亚砜,阳性对照组加入8 mmol/L的四氯化碳。培养22 h后,采用CCK-8细胞毒性实验评价木脂素的细胞毒作用,并检测培养上清GPT和GOT评价肝细胞膜通透性的改变。结果显示,醇乙在≥700μmol/L、乙素在≥100μmol/L时有细胞毒性,但均未见GPT和GOT的升高;醇甲在最高1 000μmol/L、甲素丙素在最高100μmol/L、酯甲酯乙在最高50μmol/L时均未见明显的细胞毒性。结果表明,醇乙和乙素具有一定的肝细胞毒性,主要表现为细胞代谢水平的下降,但不足以引起细胞膜通透性的显著改变;其余5种木脂素无明显肝细胞毒作用。
Schisandra chinensis has hepatoprotective effect which relys on schisandrol A, schisandrol B, schisandrin A, schisandrin B,schisandrin C,schisantherin A and schisantherin B.To investigate the toxicology of these 7 lignans,primary hepatocyte of Ctenopharyngodon idella was adopted as the cell model.1-1 000 μmol/L lignans in DMSO was added in the experimental group.The same amount of DMSO was added in the solvent control group.And 8mmol/L carbon tetrachloride was added in the positive control group.After 22 h, CCK-8 assay was employed to assess the cell toxicity,GPT and GOT were employed to assess membrane permeability change.As a result,schisandrol B showed toxicity at -〉700 μmol/L and schisandrin B at 〉 1 0 0 μmol/L.But both ol the lignans did not show GPT and GOT increasing.The other 5 lignans did not show any toxicity when adding Schisandrol A at 1 000 pmol/L, Schisandrin A and C at 100 pmol/L, and schisantherin A and B at 50 μmol/L.As a conclusion,schisandrol B and schisandrin B have mild hepatotoxicity such as metabolic inhibi-tion which do not lead to obvious GPT and GOT leakage,while the other 5 lignans do not have obvious hepato- toxicity.
出处
《江西农业大学学报》
CAS
CSCD
北大核心
2017年第3期455-459,508,共6页
Acta Agriculturae Universitatis Jiangxiensis
基金
中央级公益性科研院所基本科研业务费专项资金项目(2015C004)~~
关键词
五味子
木脂素
细胞毒性
草鱼
原代肝细胞
Schisandra chinensis
lignans
cytotoxicity
Ctenopharyngodon idella
primary hepatocyte
