摘要
目的检测一种新型硅藻土可切削陶瓷的细胞毒性,初步评价其生物相容性。方法小鼠成纤维细胞L929在新型硅藻土可切削陶瓷浸提液中培养1、3、5d,并与其他4种牙科常用修复材料作比较,MTT法观察细胞形态变化,测量吸光度(A值),计算细胞相对增殖率(RGR),判断细胞毒性的级别。结果阴性组,新型硅藻土可切削陶瓷组,铸瓷组,钴铬合金烤瓷之间无统计学差异(P>0.05)。阴性组与树脂,镍铬合金烤瓷之间存在统计学差异(P<0.05)。所有材料细胞毒性级别都在1级。结论新型硅藻土可切削陶瓷无明显的细胞毒性。
Objective To identify the cytotoxicity of a new type of diatomite-based machinable ceramic and preliminarily evaluate its biocompatibility.Methods In the present study,we used MTT to assess the cytotoxicity of the new ceramic,comparing with other four prosthodontics materials.The cell lines（L929）were treated with extract dilutions of the prosthodontics materials for 1d,3d and 5d.In order to observe the changes in cell morphology,we observed absorbance（OD value）to calculate the relative cell proliferation rate（RGR）,and determine the cytotoxicity grade.Results MTT method found no significant difference in the cell viability among control,new ceramic,pressable ceramic and Co-Cr alloy-porcelain（P0.05）.However,other groups were much higher than control（P0.05）.The cytotoxicity of all the materials was grade 1.Conclusion The new diatomite-based machinable ceramic had no apparent cytotoxicity to L929 cell.
出处
《口腔医学》
CAS
2010年第5期272-275,共4页
Stomatology
基金
江苏省国际科技合作计划资助(BZ2008059)
江苏省医学重点人才项目(RC2007099)
