摘要
目的探讨竹节参总皂苷干预自然衰老大鼠肝脏炎症的作用。方法以自然衰老大鼠为对象,将SPF级SD大鼠按月龄分为3月龄组(3 M组)、9月龄组(9 M组)、15月龄组(15 M组)、24月龄组(24 M组)及24月龄的竹节参总皂苷(TSPJ)低、中、高剂量组,每组12只大鼠。TSPJ低、中、高剂量组的大鼠从18 M开始,分别给予竹节参总皂苷10、30、60 mg·kg^(-1)灌胃至24个月,每周停药1 d,连续6个月。采用HE染色观察组织病理形态学变化,RT-PCR检测肝脏白介素-1β(IL-1β)、肿瘤坏死因子α(TNF-α)、白介素-6(IL-6)、白介素-12(1L-12)、白介素17α(IL-17α)、干扰素-γ(IFN-γ)mRNA的表达,Western blot检测肝脏IL-1β和TNF-α蛋白表达。结果随着大鼠年龄的逐渐增长,HE染色发现肝组织中肝索及肝血窦的排列紊乱加重,脂肪空泡和炎症细胞增多,TSPJ(10、30、60 mg·kg^(-1)各剂量组均能在一定程度上改善其病变情况;IL-1β、IL-6、IL-12、IL-17α、TNF-α、IFN-γmRNA和IL-1β、TNF-α蛋白的表达随着年龄的增长逐渐升高,TSPJ各剂量组均在一定程度上降低其表达。结论竹节参总皂苷对自然衰老大鼠肝脏有一定的保护作用,可能与其对抗肝脏炎症有关。
Aim To study the effects of total saponins of Panax japonicus( TSPJ) on liver inflammation of natural aging rats. Methods The experimental rats were allocated into seven groups( twelve rats in each group) : three months group,nine months group,fifteen months group, twenty-four months group, and TSPJ low-,mid-and high-dose groups( 10,30,60 mg·kg^(-1)). When the rats were eighteen months old,the TSPJ low-,mid-and high-dose groups of rats were given lavage treatments with TSPJ 10,30,60 mg ·kg^(-1)respectively until twenty-four months. During lavage,we stopped a day every week for six consecutive months. HE staining was used to observe the pathological morphological changes. Western blot was utilized to test IL-1β and TNF-α protein expressions. RT-PCR method was adopted to test IL-1β,IL-6,IL-12,IL-17α,TNF-α,IFN-γ mRNA expressions. Results HE staining observation showed that as the rats grew older the hepatic cord and sinusoid were arranged in more severe disorder,and the fat vacuole and inflammatory cells were increased significantly. While every dose group of TSPJ could improve these pathological changes distinctly. The IL-1β,TNF-α protein and IL-1β,IL-6,IL-12,IL-17α,TNF-α,IFN-γ mRNA expressions were increased gradually as the rats grew older,and every dose group of TSPJ could reduce their expressions to some extent. Conclusion TSPJ could protect the aging rat liver to some extent by inhibiting the liver inflammation.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2017年第6期848-853,共6页
Chinese Pharmacological Bulletin
基金
国家自然科学基金资助项目(No 31570329)
关键词
衰老
竹节参总皂苷
肝脏
细胞因子
炎症反应
大鼠
aging
total saponins of Panax japonicus
liver
cytokines
inflammatory responses
rat
