摘要
以‘春晨长红茄’为材料,采用不同浓度(50、100、150、200、250μmol/L)褪黑素(MT)喷施茄子幼苗叶片,以喷施清水为对照(置于自然光温下为CK,低温弱光下为CK1),研究MT对低温弱光胁迫下茄子幼苗生理特性的影响。结果表明:浓度低于200μmol/L的MT能保持低温弱光胁迫下茄子幼苗叶片中叶绿素和类胡萝卜素含量,当MT浓度为150μmol/L,茄子幼苗叶片叶绿素总量和类胡萝卜素含量分别比CK1高40.23%和75.00%;喷施MT,有助于提高PSII活性,增大光能捕获和转化效率;喷施MT能提高茄子幼苗叶片POD、SOD、CAT活性,降低MDA含量,促进脯氨酸和可溶性糖的积累,当MT浓度为150μmol/L时,与CK1相比,茄子幼苗叶片POD活性提高了108.66%,可溶性糖含量提高了50.61%。综合本研究结果,喷施MT能提高茄子幼苗叶片对低温弱光的抵抗力,且浓度为150μmol/L时效果较好。
In this study, five different concentrations （50, 100, 150, 200, 250 pmol/L） of melatonin （MT） were sprayed to the eggplant seedlings under the stress of low light and low temperature, CK and CK1 were sprayed with fresh water but the CK was under natural light and CK1 was under the stress of low light and low temperature, to study the effects of different concentrations of MT on the physiological characteristics of eggplant seedlings under the stress of low light and low temperature. The results showed that photosynthetic pigment content, PSII photochemistry, and efficiency of light energy conversion and rate of photosynthesis in eggplant seedling kept at high level when the concentration of melatonin was below 200 μmol/L; when the MT concentration was 150 μmol/L, the total chlorophyll and carotenoid content of eggplant were 40.23% and 75.00% higher respectively than those of CK1; the activities of antioxidase （POD,SOD,CAT） increased significantly and the POD activity was 108.66% higher than CK1; the accumulations of proline and soluble sugar were accelerated and the soluble sugar content was 50.61% higher than CK1, while the content of malonaldehyde was reduced in leaves of eggplant seedling. Thus, exogenous melatonin can enhance the resistance to the stress of low light and low temperature of eggplant seedling, and the best concentration is 150 μmol/L
出处
《湖南农业大学学报（自然科学版）》
CAS
CSCD
北大核心
2017年第3期257-261,共5页
Journal of Hunan Agricultural University(Natural Sciences)
基金
四川省教育厅科研项目(15ZA0011)
四川农业大学"双支"计划项目(03571863)
关键词
茄子幼苗
褪黑素
低温弱光
生理特性
叶绿素荧光
eggplant seedlings
melatonin
low irradianee and low temperature
physiological properties
chlorophyll fluorescence parameters
