摘要
目的:考察健脾生血颗粒对围产期大鼠的毒性作用。方法:将受孕大鼠按体质量随机分为阴性对照组和健脾生血颗粒低、中、高剂量组(0.77、2.31、6.93 g/kg),每组21只,从妊娠第15天开始至分娩后第21天止每天ig相应药物1次。观察健脾生血颗粒对母代大鼠一般毒性和生育能力的影响,对F1代仔鼠运动能力、学习记忆能力、生育能力以及对F2代仔鼠早期发育能力的影响。结果:对母代的毒性作用方面,健脾生血颗粒高剂量组母代大鼠妊娠天数较阴性对照组显著延长(P<0.05),给药初期体质量显著降低(P<0.01)。对子代的毒性作用方面,健脾生血颗粒高剂量组F1代仔鼠初生体质量较阴性对照组显著降低(P<0.05),第4、5次发现水下平台时间较阴性对照组显著缩短(P<0.01);对F1代仔鼠生育能力及F2代仔鼠早期发育能力均未见明显影响(P>0.05)。健脾生血颗粒中、低剂量组子代大鼠各指标均未见受到明显影响。结论:健脾生血颗粒对母代及子代大鼠均未见毒性反应的剂量为2.31 g/kg。
OBJECTIVE： To investigate the toxicity effect of Jianpi shengxue granule on perinatal rats. METHODS： Based on body mass, pregnant rats were randomly divided into negative control group and Jianpi shengxue granule low-dose, medium-dose, high-dose groups （0.77, 2.31, 6.93 g/kg）, 21 in each group, and intragastrically given relevant medicines from the 15th d of preg- nancy until 21st d after delivery, once a day. Effects of Jianpi shengxue granule on general toxicity and fertility of maternal rats were observed, as well as the effects on athletic ability, learning and memory ability, fertility of F1 offspring and early viability of F2 offspring. RESULTS： In terms of toxicity effect on maternal rats, compared with control group, the days of pregnancy in Jianpi shengxue granule high-dose group was significantly prolonged （P〈0.05）, initial body mass of administration was significantly re- duced （P〈0.01）. In terms of toxicity effect on offspring, compared with negative control group, the initial body mass of F1 off- spring in Jianpi shengxue granule high-dose group was significantly reduced （P〈0.05）, the time of 4th and 5th time for founding underwater platform was significantly shortened （P〈0.01）, and there was no obvious effect on fertility of F1 offspring and early vi- ability of F2 offspring （P〉0.05） ; there was no obvious effect on the indexes of offspring in Jianpi shengxue granule medium- dose, low-dose groups. CONCLUSIONS： The no toxic dose of Jianpi shengxue granule in maternal and offspring rats is 2.31 g/kg.
出处
《中国药房》
CAS
北大核心
2017年第16期2190-2193,共4页
China Pharmacy
基金
武汉市汉阳区"汉阳英才计划"项目(No.A3-17)
