摘要
目的评价外源性NO对小鼠卵母细胞体内成熟、体外受精及胚胎发育的影响。方法受试雌性小鼠超排卵期间分别腹腔注射NO供体硝普钠(SNP)1.0、5.0和10.0 mg/kg,对照组雌性小鼠注射等量的生理盐水。注射hCG 15 h后处死小鼠,收集卵母细胞进行体外受精和胚胎培养,观察卵母细胞的成熟度、受精率、早期胚胎的发育能力及其形态学变化。结果5.0 mg/kg SNP组M期卵母细胞和M期卵母细胞的比例较对照组和1.0 mg/kg SNP组升高(P<0.05,P<0.01),而受精率降低(P<0.01),10.0 mg/kg SNP组小鼠在注射药物20min后全部死亡;5.0 mg/kg SNP组2-细胞胚胎以后各阶段胚胎的卵裂率低于对照组和1.0 mg/kg SNP组(P<0.01),并被阻滞在桑葚胚期;5.0 mg/kg SNP组形态异常胚胎的比例高于对照组和1.0 mg/kg SNP组(P<0.01)。结论较高浓度NO抑制卵母细胞体内成熟、体外受精及早期胚胎发育并影响胚胎的质量。
Objective To evaluate the effects of exogenous nitric oxide on mouse oocyte maturation in vivo,in vitro fertilization and embryo development.Methods By intraperitoneal injection,the experimented female mice were administered separately with sodium nitroprusside（SNP） of dosages at 1.0 mg/kg,5.0 mg/kg and 10.0 mg/kg when stayed in period of superovulation,and at the same time,the control mice were administered with equal volume of saline solution.At 15 hours after injected intraperitoneally with hCG,the mice were killed by cervical dislocation,of which the oocytes in the oviducts were collected for in vitro fertilization,and also the oocyte maturation,the embryo development and morphological change were observed.Results ① In 5.0 mg/kg SNP group,the rates of MI and MII oocytes with no fertilization were higher than those in the control group and 1.0 mg/kg SNP group（P〈0.05,P〈0.01）,but the fertilization rate of oocytes was lower in 5.0 mg/kg SNP group than in the control group and 1.0 mg/kg SNP group（P〈0.01）,all mice in 10.0mg/kg of SNP group died of 20 minutes after administration.② The cleavage rate of embryos after 2-cell stage was lower in 5.0 mg/kg SNP group when compared separately with that in the control group and 1.0 mg/kg SNP group（P〈0.01）,none of the embryos developed to blastocyst stage in 5.0 mg/kg SNP group.③ The rate of the fragment formation in embryos during progress was higher in 5.0 mg/kg SNP group than in the control group and 1.0 mg/kg SNP group（P〈0.01）.Conclusion The higher concentration of nitric oxide inhibits oocyte maturation in vivo,in vitro fertilization and embryo development,and affects the embryo quality.
出处
《四川大学学报（医学版）》
CAS
CSCD
北大核心
2007年第5期799-801,共3页
Journal of Sichuan University(Medical Sciences)
基金
贵州省重点攻关项目(计字2001-1214)
合肥市科委项目(遵市科合社字2002-06号)资助
关键词
NO
卵母细胞
体外受精
胚胎
发育
Nitric oxide Oocyte maturation in vitro fertilization Embryo Development
