摘要
目的:探讨软骨分化的间充质干细胞对颞下颌关节炎软骨修复的影响。方法:将诱导出骨关节炎模型4周后的新西兰白兔46只,关节内分别注射hylartin溶液(安慰剂组,A组)、非软骨分化的间充质干细胞(B组)、软骨分化的间充质干细胞(C组)。注射后的4、12、24周观察软骨的组织学变化。结果:与第4周相比,术后第12周软骨分化间充质干细胞组的软骨分化有较好的组织学表现,但这一差别在第24周时变小。术后第4周,可在软骨及关节囊中检测到注射的细胞。结论:关节腔内注射间充质干细胞可延缓颞下颌关节炎软骨病变的进展,且体外软骨向诱导的间充质干细胞可在更大程度上提高其治疗效果。
Objective:This study investigated the effects of chondrogenic differentiated mesenchymal stem cells(MSCs)on cartilage in temporomandibular joint osteoarthritis(TMJOA)in vitro.Methods:Four weeks after induction of osteoarthritis(OA),the joints received hylartin solution,non-chondrogenic MSCs or in vitro chondrogenie differentiated MSCs.The changes in cartilage were evaluated by histology,reverse transcription polymerase chain reaction.Results:The differentia-ted MSC-treated group had better histology than the MSC-treated group at 4 and 12 weeks,but with no difference at 24 weeks.Transplanted cells were observed at cartilage and the synovial membrane lining at 4 weeks.Conclusion:Intraarticular injection of MSCs could delay the progression of TMJOA,and in vitro chondrogenic induction of MSCs could enhance the therapeutic effects.
作者
王鹏
陈瞰
任笑春
叶斌
李运峰
祝颂松
WANG Peng;CHEN Kan;REN Xiao-chun;YE Bin;LI Yun-feng;ZHU Song-song(State Key Laboratory of Oral Diseases,National Clinical Research Center for Oral Diseases,West China Hospital of Stomatolo-gy,Sichuan University,Sichuan Chengdu 610041,China)
出处
《临床口腔医学杂志》
2017年第4期203-207,共5页
Journal of Clinical Stomatology
基金
国家自然科学基金资助(81271170)
关键词
骨髓间充质干细胞
软骨细胞分化
颞下颌关节
骨性关节炎
软骨
Mesenchymal stem cells
Chondrogenic differentiation
Temporomandibular joint
Osteoarthritis'
Cartilage
