摘要
通过比较有、无诱导条件下正常与骨质疏松症(OP)大鼠骨髓间充质干细胞(MSCs)骨向、脂向及软骨向的分化情况,观察去卵巢对SD大鼠MSCs多向分化能力的影响。实验分为四组:正常组、正常诱导组、OP组、OP诱导组;分别在有或无成骨、成脂、成软骨诱导条件下,评价各组的分化情况。检测发现,无诱导条件下,正常大鼠MSCs骨向及软骨向分化的能力强于OP大鼠,而OP大鼠MSCs的脂向分化能力强于正常大鼠;诱导条件下,正常大鼠MSCs对骨向及软骨向诱导剂的反应能力强于OP大鼠,而OP大鼠MSCs对成脂诱导剂的反应能力强于正常大鼠。这些结果表明,去卵巢后大鼠MSCs骨向分化能力及对成骨诱导剂的反应力下降,脂向分化能力及对成脂诱导剂的反应力增强,对软骨诱导剂的反应力下降。
To investigate the effects of ovariectomy on the ability of multidirectional differentiation into osteoblast,adipocyte and chondrocyte of Sprague-Dawley(SD) rats’ mesenchymal stem cells(MSCs) was compaired by the differentiatial condition of the MSCs derived from the osteoporosis(OP) rats and normal rats with and without induced conditions.The experiment was divided into four groups: normal group,normal induced group,OP group,and OP induced group.The ability of multidirectional differentiation was detected after treated with or without osteoblast-induced,adipocyte-induced or chondrocyte-induced conditions,respectively.The results demonstrated that the ability of osteogenic and chondral differentiation of MSCs from normal rats was stronger than that from OP rats without the induced conditions,but the ability of adipose differentiation was lower.With the induced conditions,the reaction to osteoblast-induced and chondrocyte-induced agent of MSCs from normal rats was stronger than that from OP rats,but the reaction to adipocyte-induced agent was lower.In conclusion,compared with MSCs from normal rats,the ability of osteogenic differentiation and the reaction to osteoblast-induced agent of MSCs from OP rats decreased,the ability of differentiation into adipocyte and reaction to chondrocyte-induced agent of MSCs from OP rats increased,and the reaction to chondrocyte-induced agent decreased.
出处
《中国细胞生物学学报》
CAS
CSCD
北大核心
2012年第1期61-66,共6页
Chinese Journal of Cell Biology
基金
国家自然科学基金(No.30772885)
国家中医药管理局中医药科学研究专项(No.04-05JL19)资助项目~~
关键词
骨髓间充质干细胞
大鼠
骨质疏松
多向分化
mesenchymal stem cells
rats
osteoporosis
multidirectional differentiation
