摘要
目的:通过实验探讨透明质酸钠预防微波消融术后局部组织粘连的效果。方法:120只健康SD大鼠随机分为三组,每组40只,分别为微波消融组(A组,对照组)、微波消融加透明质酸钠组(B组)、微波消融加生理盐水组(C组)。通过对大鼠背部肌肉进行微波消融建立术后局部粘连模型,并在该模型基础上应用透明质酸钠,术后3 d、1 w、2 w、4 w分别对背部组织进行粘连分级、HE染色及免疫组化观察和分析。结果:消融术后3 d、1 w、2 w,B组在粘连分级方面A、B及C 3组间水平差异具有统计学意义P<0.05);4 w时A、B、C组间的差异无统计学意义(P=0.458)。两两比较,A、C两组在四个时间点水平差异无统计学差异(P均>0.05),而B组在消融术后3 d、1 w和2 w时,粘连分级明显优于A、C组,P=0.029(3 d)、P=0.011(1W)、P=0.004(2W),水平差异显著,有统计学意义;4 w时,P=0.391,无统计学意义。消融术后A、C组HE染色及免疫组化染色光镜下观察,淋巴细胞、巨噬细胞等炎症浸润情况及成纤维细胞数目均较B组多,且A、C组胶原纤维沉积明显多于B组。结论:透明质酸钠能够有效减轻微波消融术后发生的局部组织粘连。
Objective：To determine the effectiveness of sodium hyaluronate in preventing local tissue adhesion after microwave ablation.Methods：120 rats were randomly divided into three groups （40 rats in each group）：microwave ablation group （group A）,microwave ablation and sodium hyaluronate group （group B）,microwave ablation and sodium hyaluronate saline group （group C）.The local adhesion model was established in muscle of rat back by microwave ablation,and sodium hyaluronate was applied in this model during operation.Biopsy was taken on the 3th,7th,14th and 28th postoperative day.The adhesion scores gradation judging,hematoxylin and Immunohistochemistry were observed and analyzed.Results：There were statistically significantly differences in the extent of adhesion between group B and group A,C on the 3th,7th and 14th postoperative（p〈0.05）,after ablation 4W,there was not statistically signifi-cantly differences among A,B,C three groups （P=0.458）.Comparison between the two group,there was no statistical significance be-tween the group A and C in the four time points （P〉0.05）,however,on the 3th,7th and 14th postoperative day,group B were significantly less than group A,C in the extent of adhesion,and there was statistical significance,P=0.029（3d）,P=0.011 （1w）,P=0.004（2w）.After ablation 4w,P=0.391,there was no statistical significance between group B and group A,C.Group A and C were more than group B in the aspect of hematoxylin and immunohistochemical staining,the number of inflammatory cells infiltrated by lymphocytes,fibroblasts and macrophages,and the amount of collagen fiber deposition in A and C group were significantly harder than group B.Conclusion：Sodium hyaluronate appears to effectively reduce the local tissue adhesion after microwave ablation.
出处
《现代生物医学进展》
CAS
2017年第15期2837-2842,共6页
Progress in Modern Biomedicine
基金
国家自然科学基金项目(81171436)
关键词
透明质酸钠
微波消融
粘连
预防
Sodium hyaluronate
Microwave ablation
Adhesion
Preventing
