摘要
目的 了解基膜黏连蛋白和Ⅳ型胶原对体外培养条件下许旺细胞的贴壁及增殖作用的影响。 方法 用基膜黏连蛋白和Ⅳ型胶原包被培养板 ,以结合了相应抗体的培养板及包被Ⅰ型胶原和多聚赖氨酸的培养板为对照 ,将培养的许旺细胞以相同浓度加入各组培养板内。测定 2、6、2 4h细胞贴壁率 ,培养 72h进行3 H TdR掺入试验 ,双道液体闪烁计数器测定各组的放射性计数。 结果 基膜黏连蛋白和Ⅳ型胶原组早期贴壁率较Ⅰ型胶原和多聚赖氨酸组高 ,抗基膜黏连蛋白和抗Ⅳ型胶原组贴壁率较低。基膜黏连蛋白和Ⅳ型胶原组3 H TdR掺入量高于其它组。
Objective To detect the effects of laminin and type Ⅳ collagen on the attachment and proliferation of Schwann cells Method The plated were covered by laminin and collagen Ⅳ, the control groups were added antibody of laninin and collagen Ⅳ, collagen Ⅰ and polylysine The same concentration of Schwann cells were cultured in each groups plates The rates of cells attachment of each group were determined after 2,6,24 hours After 72 hours 3*'H TdR were mixed into culture matrix fluid and the cpm were mensurate Results The attachment rates of Laminin and collagen Ⅳ groups were higher than that of the control groups and the amount of mixed 3*'H TdR were also higher than that of control groups Conclusion Laminin and collagen Ⅳ promote the attachment and proliferation of Schwann cells
出处
《中华显微外科杂志》
CSCD
北大核心
2002年第3期194-196,共3页
Chinese Journal of Microsurgery
