摘要
目前在小麦中虽然已经命名了20余个矮秆基因,但小麦矮秆基因资源应用单一化的现状仍然存在,因此对小麦新矮源的筛选与研究显得十分必要。本研究以1个小麦矮秆突变体‘矮128’为材料,通过赤霉酸处理、遗传分析、基因等位性测验和DNA分子标记等手段分析了该矮秆突变体矮秆基因的性质及可能来源。结果表明:‘矮128’属赤霉酸不敏感型矮秆突变体,其矮秆性状受1对隐性基因控制,该基因与Rht8、Rht9、Rht13、RhtB1b(Rht1)、RhtD1b(Rht2)、RhtD1c(Rht10)和Rht16等矮秆基因不是等位基因,也不同于Rht4、Rht5、Rht8、Rht9、Rht12、Rht13等6个已知矮秆基因。尽管如此,‘矮128’中的矮秆基因是否为新的矮秆基因仍然需进一步的遗传分析加以明确。
More than twenty redueed-height（Rht）（or dwarfing） genes have been designated in wheat by far, however, only few Rht genes were successfully used in wheat breeding pro- grams,thus making the genetic diversity of Rht genes in wheat very narrow. Therefore,it is of great importance to screen and make use of new Rht genetic resources for future necessity of wheat breeding programs. A dwarf wheat mutant（＇Ai 128＇）was analyzed in present study with gibberellin treatment,genetic analysis,allelism test and molecular marker assay to deter- mine the nature and putative origin of the Rht gene in＇Ai 128＇. The results showed that the dwarfing mutant is gibberellin-insensitive,and the dwarfing phenotype was controlled by one pair of recessive genes. Allelism test confirmed that the gene conferring the dwarfing trait in ＇ Ai 128 ＇ was not allelic to Rht8 ,Rht9 ,Rhtl 3 ,RhtBlb （Rhtl） ,RhtDlb （Rht2） ,RhtDlc （Rhtl 0 ） and Rht16 ,and was also different to Rht4 ,Rht5 ,Rht8 ,Rht9 ,Rht12 and Rhtl3, assayed usingDNA markers linked with these known Rht genes. Nevertheless, whether the Rht gene in＇Ai 128＇is a new gene or not needs to be determined by further genetic analysis,such as construc- tion of high-density molecular map using segregant populations derived from crosses of＇Ai 128 ＇with other taller-height wheat lines.
作者
张祥池
郝燕冉
魏凡
张志锋
魏佳佳
付帅
安浩军
王睿辉
ZHANG Xiang-chi HAO Yan-ran WEI Fan ZHANG Zhi-feng WEI Jia-jia FU Shuai AN Hao-jun WANG Rui-hui(College of Agronomy, Agricultural University of Hebei, Baoding 071001,China Baoding Academy of Agricultural Sciences,Baoding 071001 ,China)
出处
《河北农业大学学报》
CAS
CSCD
北大核心
2017年第2期1-6,23,共7页
Journal of Hebei Agricultural University
基金
河北农业大学大学生创新创业训练计划资助项目(20160104
20161008019)
河北农业大学"百名青年学术带头人培养项目"
河北省现代农业产业技术体系小麦创新团队等项目资助
关键词
小麦
矮秆突变体
矮秆基因
赤霉酸反应
分子标记
基因等位性测验
wheat（Triticurnaestivurn L. ）
dwarfing mutant
reduced height genes
gibberel- lin reaction~ molecular marker
allelism tests
