摘要
目的:通过观察黄芪总苷对TGF-β1诱导足细胞TRPC6表达的影响,探讨黄芪总苷对足细胞保护作用。方法:体外培养小鼠肾小球足细胞,将成熟的足细胞分为5组:正常对照组、TGF-β1干预组、TGF-β1干预+低剂量黄芪总苷组、TGF-β1干预+中剂量黄芪总苷组、TGF-β1干预+高剂量黄芪总苷组,48 h后MTT检测各组细胞增殖抑制率,Western blot及RT-PCR检测TRPC6蛋白及mRNA表达水平。结果:TGF-β1干预使足细胞足突回缩、甚至消失,抑制足细胞的增殖(P<0.05),提高TRPC6mRNA和蛋白表达(P<0.05),黄芪总苷能逆转上述变化,对足细胞具有保护作用并且存在一定的量效关系。结论:TPRC6在TGF-β1干预下足细胞损伤作用中起重要作用,黄芪总苷可能通过下调足细胞TRPC6的表达实现对足细胞的保护作用。
Objective： To explore the possible mechanism of astragaloside involved in the mouse podocytes injury induced by TGF-β1 in vitro. Methods： Mouse podocytes were cultured in vitro and then all cell were divided into 5 groups： normal control group,TGF-β1 treatment group,TGF-β1 treatment ＋astragaloside low dose group,TGF-β1 treatment ＋astragaloside middle dose group and TGF-β1 treatment ＋astragaloside high dose group. The proliferation rate of each group was investigated by MTT assay,the expression of TRPC6 protein and mRNA were measured by Western blot and RT-PCR respectively after 48 hours. Results： TGF-β1 can significantly inhibit the proliferation of podocytes（ P〈0. 05）,fusions of foot processes or even effaced of podocytes were observed. TGF-β1 could also increase the expression of TRPC6. Astragaloside could reduce the inhibition of TGF-β1 to the proliferain of podocytes significantly,make the cell shape tend to be normal,and reduce the expression of TRPC6 mRNA and protein with dose-effect relation. Conclusion： TRPC6 play an important role in the TGF-β1 induecd podocytes injury. Astragaloside can alleviate podocytes injury by reduce the expression of TRPC6.
作者
黄海庭
吴好好
覃幼玲
林栩
尤燕舞
郭鹏威
汤春荣
HUANG Hai-Ting WU Hao-Hao QIN You-Ling LIN Xu YOU Yan-Wu GUO Peng-Wei TANG Chun-Rong(Department of Nephrology, the Affiliated Hospital of Youjiang Medical University for Nationalities, Baise 533000, Chin)
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2017年第3期370-373,共4页
Chinese Journal of Immunology
基金
广西自然科学基金(2014GXNSFAA118269)
