摘要
目的:动态观察黄芪(AM)对单侧输尿管梗阻(UUO)所致大鼠肾小管问质纤维化的拮抗作用及对转化生长因子β1(TGF-β1)表达的影响,初步探讨其可能的分子作用机制。方法:SD大鼠随机分为假手术组、UUO模型组和黄芪治疗组。在造模后3,7,14 d处死动物。观察肾脏病理改变。采用免疫组化方法检测肾组织中TGF-β1和α-平滑肌肌动蛋白(α-SMA)表达。用实时荧光定量RT-PCR检测大鼠TGF-β1 mRNA和α-SMA mRNA表达量。用Western blot检测大鼠TGF-β1蛋白表达量。结果:与假手术组相比,各时间点的UUO大鼠肾脏病理损害进行性加重,TGF-β1和α-SMA mRNA和蛋白表达随梗阻时间延长逐渐增高;黄芪治疗后可明显减轻UUO大鼠肾组织的肾小管损伤及肾间质纤维化,使UUO大鼠肾组织中高表达的TGF-β1和α-SMA降低(P<0.05)。结论:黄芪可能通过从核酸和蛋白水平抑制TGF-β1的表达,抑制肾小管上皮细胞转分化,减轻和改善UUO大鼠肾间质纤维化。
Objective:To study the effect of Astragalus mongholicus(AM) on the expression of transforming growth factor-β1 (TGF-β1) in SD rats with unilateral ureteral occlusion (UUO) and to elucidate the mechanisms underlying the renoprotective effects of AM.Method:Fifty-four Spragne-Dawley rats were randomly divided into 4 groups:sham-operation group,the UUO group and AM treatment group.After administration of AM (10 g·kg^(-1)·d^(-1)) for 3,7 and 14 days,the dynamic histological changes of renal interstitial tissues ...
出处
《中国中药杂志》
CAS
CSCD
北大核心
2009年第2期193-198,共6页
China Journal of Chinese Materia Medica
关键词
黄芪
转分化
转化生长因子Β1
单侧输尿管梗阻
Astragalus mongholicus
transdifferentiation
transforming growth factor-β1
unilateral ureteral occlusion
