摘要
目的对分离纯化得到的制黄精多糖PSP-1-A进行体外抗氧化活性测试。方法制黄精经分离纯化后得到制黄精粗多糖(PSP)及PSP-1-A,采用体外抗氧化实验法测定PSP及PSP-1-A清除2,2-二苯基-1-苦肼基(DPPH)自由基、对羟基自由基的能力及总抗氧化能力。结果测得PSP及PSP-1-A在体外均能明显清除DPPH自由基、对羟基自由基和ABTS+自由基,且其体外抗氧化功能在一定范围内随浓度的增大而增大。结论 PSP及PSP-1-A在一定浓度下均具有明显的体外抗氧化作用。
Objective To research the in vitro antioxidative effect of separated and purified polysaccharide PSP-1-A from Polygonatum Sibiricum Redoute.Methods The polysacchrade PSP-1-A was obtained from Polygonatum Sibiricum Redoute by separation and purification.The in vitro antioxidative experimental method was adopted to determine the ability of PSP and PSP-1-A for clearing DPPH free radical and hydroxyl free radical,and total antioxidant capability.Results It was detected that PSP and PSP-1-A could obviously clear in vitro DPPH free radical,hydroxyl radical and ABTS＋radical,moreover its in vitro anti-oxidative effect was increased with its concentration increase in some range.Conclusion PSP and PSP-1-A have significant antioxidative activity in vitro under a certain concentration.
出处
《检验医学与临床》
CAS
2017年第6期776-777,780,共3页
Laboratory Medicine and Clinic
关键词
黄精
多糖
抗氧化
Polygonatum Sibiricum Redoute
polysaccharide
anti-oxidation
