摘要
目的:观察六味地黄汤对慢性肾衰大鼠肾小管上皮细胞EMT的影响。方法:将SD大鼠随机分为正常组、假手术组、模型组、六味组,采用5/6肾切除法复制模型,用免疫组化、Western Blot、RT-PCR检测留存肾E-cadherin、α-SMA、FSP-1在蛋白和mRNA水平表达差异。结果:模型组E-cadherin下调,α-SMA、FSP-1上调,以上组间差异有统计学意义(P<0.01),出现EMT现象;与模型组比,六味组E-cadherin上调,α-SMA、FSP-1下调,EMT现象减轻。结论:大鼠5/6肾切除致慢性肾衰模型留存肾中存在EMT现象;六味地黄汤可抑制EMT。
Objective： To observe the effects of Liu Wei Di Huang Decoction on renal tubular epithelial cell EMT of chronic renal failure. Methods： SD rats were randomly divided into normal group, sham operation group, model group, Liuwei group. Copy 5/6 nephrectomy rat model of chronic renal failure, Immuuohistochemistry, Western blot, RT-PCR methods were used to detect E-cadherin, α-SMA, FSP-1 expression differences of the rat remnant kidney at the protein and mRNA levels. Results： Model group in the protein and mRNA levels, E-cadherin expression level was down-regulated,α- SMA and FSP-1 expression were up-regulated, the difference between the above group were statistically significan （P 〈 0. 01 ）, EMT phenomenon appears; Compared with model group： In Liuwei group of rats E-cadherin expression was up, α- SMA, FSP-1 expression were down, EMT phenomena reduced. Conclusion： EMT phenomenon in the rat remnant kidney of 5/6 nephrectomy-induced chronic renal failure model; The Liuwei Dihuang decoction inhibit the EMT.
出处
《中国中医基础医学杂志》
CAS
CSCD
北大核心
2014年第1期47-51,89,共6页
JOURNAL OF BASIC CHINESE MEDICINE
基金
湖南省科技计划基金项目-5/6肾切除大鼠肾小管上皮细胞转分化及六味地黄丸的干预研究(2011FJ3007)
湖南省中医药管理基金项目-从肾胚胎形成角度探讨六味地黄汤对慢性肾衰竭大鼠的作用(2011042)
湖南省研究生科研创新项目-慢性肾衰大鼠干细胞修复机理及六味地黄丸干预研究(CX2010B337)
湖南中医药大学校级教改项目-以学生为本组建兴趣小组法提高中医院校病理学教学效果探讨(2011-JG005)
关键词
六味地黄汤
慢性肾衰竭
肾小管上皮细胞
EMT
Liu Wei Di Huang Decoction
Chronic renal failure
Renal tubular epithelial cell
EMT
