摘要
目的:初步评价应用于口腔正畸支抗种植体的金属材料:商业纯钛(cpTi),钛合金(Ti_6Al_4V),医用316L不锈钢的生物安全性。方法:参考国标GB/T16886.5-2003以及医疗行业标准YY/T0127.2-2009所规定的方法,采用体外细胞毒性实验,体外遗传毒性实验及急性全身毒性实验对3种材料进行测试。结果:cpTi、Ti_6Al_4V和平3 16L不锈钢100%浸提液的细胞毒性分别为0级、1级、2级;DNA OTM分别为2.16±0.09,9.84±1.78和10.79±0.39;细胞尾部DNA分别为(0.43±0.01)%、(0.92±0.43)%和(1.22±0.06)%。小鼠体内急性毒实验3种材料显示毒性体征,未引起小鼠体重变化。结论:cpTi生物安全性优于Ti6A14V和316L。
Objective： To evaluate the biological safety of commercial pure Ti（ cpTi）, Ti alloy Ti6A14V and stainless steel 316L Methods： According to the GB/T16886.5-2003 and YY/T0127.2-2009 standards, the extraction of the 3 materials was respectiyely prepared, cck-8 assay was used to test their cytotoxicity, single cell gel eleetrophoresis was used to test their genotoxicity, and in vivo test was used to test their acute systemic toxicity. Results： The cytotoxicity of 100% extractions of 316L, Ti6Al4V and epTi was gra- ded to 2, 1 and 0, the olive tal moment（OTM） 10.79 ±0.39, 9.84 ±1.78 and 0.92 ± 1.43, the percentage of tail DNA （ 1.22 ± 0.06） % , （0.92±0.43 ）% and （0.43 ± 0.01 ）% respectively. No systemic toxicity and no body weight change was observed in the acute systemic toxicity test in mice. Conclusion： cpTi has better biologic safety than Ti6 Al4 V and 316L.
出处
《实用口腔医学杂志》
CAS
CSCD
北大核心
2017年第2期179-183,共5页
Journal of Practical Stomatology
