摘要
[目的]评价不同粒重、种皮颜色和生态类型大豆材料中皂醇含量的差异性,筛选高皂醇含量基因型大豆材料,为大豆高皂醇含量品种选育和机理研究提供理论依据。[方法]以具有代表性生态类型的72个大豆品种(系)为材料,用HPLC法对大豆皂醇含量进行了测定。[结果]供试大豆品种总皂醇含量变异范围为0.92~4.02mg·g^(-1);小粒型材料皂醇A、皂醇B和总皂醇含量显著高于中粒型和大粒型材料;黑色种皮大豆材料皂醇A和总皂醇含量显著高于黄色和绿色;野生大豆材料皂醇A和总皂醇含量显著高于栽培种;不同组织胚中皂醇A、皂醇B和总皂醇含量最高,种皮最低。[结论]不同大豆品种皂醇含量差异显著,且小粒、黑色、野生大豆材料中皂醇含量最高。
[Objective]To provide theoretical basis for the selection and mechanism study of soybean varieties with high content of soyasapogenol,the difference of soyasapogenol contents in different grain weight,seed coat color and ecological type of soybean materials were evaluated,soybean genotype materials with high soyasapogenol content were screened.[Methods]The contents of soyasapogenol were determined by high performance liquid chromatography（HPLC）method with 72 soybean varieties from Shanxi as materials.[Results]We found that the soyasapogenol contents of 72 soybean varieties ranged from 0.92 to 4.02mg·g-1;the contents of soyasapogenol A,B and the total soyasapogenol of small grain materials were significantly higher than those of medium grain and large grain type materials;the soyasapogenol A and total soyasapogenol contents of soybean varieties with black seed coat were significantly higher than those with yellow or green seed coat;the contents of soyasapogenol A and the total soyasapogenol in the wild soybean were significantly higher than those in the cultivated species;In different tissues,the content of soyasapogenol A,B and total soyasapogenol in hypocotyl was the highest,and the content of soyasapogenol in seed coat was the lowest.[Conclusion]The content of soyasapogenol was significantly different in different soybean varieties,the content of soyasapogenol in the variety with the characteristics of small grains,black seed coat,wild materials was the highest.
出处
《山西农业大学学报（自然科学版）》
CAS
2017年第3期201-206,213,共7页
Journal of Shanxi Agricultural University(Natural Science Edition)
基金
山西省科技计划项目(20120311005-3)
山西省高等学校科技创新项目(2015148)
山西省自然科学基金(201601D011076)
关键词
大豆
皂苷
皂醇
HPLC
Soybean
Saponin
Soyasapogenol
HPLC
