摘要
目的 :观察基因 ras和 p16在肝癌组织中的表达 ,探讨其在肝癌术后复发及预后中的作用。方法 :应用免疫组化 S- P法 ,检测 5 3例手术切除肝癌组织和 12例正常肝组织的 ras和 p16表达及与肝癌临床病理指标、术后复发及预后的关系。结果 :(1) ras在肝癌和正常肝组织中的阳性表达率分别为 6 6 .0 %和 34.0 % ,ras表达与肿瘤大小、有无肝内转移密切相关 (P均 <0 .0 5 )。ras阳性、阴性表达病例 3年复发率分别为 6 8.8%、38.9% ,3年生存率分别为37.1%、6 6 .7% (P<0 .0 5 )。 (2 ) p16在肝癌和正常肝组织中的阳性表达率各为 4 7.2 %、10 0 .0 % ,p16表达与有无肝内转移、组织分化密切相关 (P均 <0 .0 5 )。 p16阳性、阴性表达病例 3年复发率分别为 4 8.0 %、6 7.9% ,其 3年生存率分别为 6 4 .0 %、32 .1% (P均 <0 .0 5 )。 (3) ras和 p16在肝癌中的表达密切相关 (P<0 .0 1) ,p16阳性 ras阴性与 p16阴性ras阳性表达病例 3年复发率分别为 35 .7%、79.2 % ,3年生存率分别为 71.4 %、2 9.2 % ,差异有极显著性 (P<0 .0 1)。结论 :联合检测 p16和 ras的表达可作为判断肝癌术后复发及预后的有效指标 。
Objective:To approach ras and p16 protein expression in hepatocellular carcinoma .To investigate their effect on recurrence and prognosis of hepatocellular carcinoma after hepatectomy.Methoeds:Ras and p16 protein expression in 53 cases of human hepatocellular carcinoma and in 12 cases of normal liver were examined by using immunohistochemical technique,respectively.Results:(1)Positive staining rate of ras in hepatocellular carcinoma and in normal liver was 66.0% and 34.0%(P<0.05),respectively.The positive expression of ras correlated with tumor size,intrahepatic metastsis,recurrence and survical rate after hepatectomy(P<0.05).(2)Positive staining rate of p16 was more higher in normal liver than in hepatocellular carcinoma(P<0.01).The negative expression of p16 correlated with phthological grading ,intrahepatic metastasis and prognosis(P<0.05).(3)There was a significant correlation between ras and p16 protein expression (P<0.01).The 3-year recurrence and survival rate was 35.7% and 71.4% respectively in the opposites.Conclusions: Protein expressions of ras and p16 are effective in dicators for recurrence and prognosis of hepatocellular carcinoma.The analysis of multi-gene is more valuable than that of single-gene. [
出处
《南通医学院学报》
2002年第3期246-248,共3页
ACTA Academiae Medicinae Nantong
