摘要
目的:本文对制何首乌精准煮散饮片用药形式进行探索研究。方法:比较制何首乌饮片及煮散饮片的出膏率;比较3批制何首乌饮片及采用相同饮片制备的煮散饮片质量的均一性,并对其化学指纹图谱进行相似度评价,采用相对峰面积比较共有峰含量和质量均一性。应用ITS2序列作为DNA条形码对何首乌原药进行鉴定。结果:制何首乌精准煮散饮片出膏率为原饮片的2.5倍;3批原饮片煎煮液二苯乙烯苷平均含量为3.56±2.61 g·mg-1,RSD为73.28%,煮散饮片煎煮液二苯乙烯苷平均含量为13.23±0.37 g·mg-1,RSD为2.82%;煮散饮片与原饮片的指纹图谱相似度基本一致,但煮散饮片各共有峰的含量、均一性明显提高。ITS2序列对何首乌药材可实现准确鉴定。结论:制何首乌煮散饮片显著提高煎煮率及质量均一性,可节约资源,提高临床疗效。
This study aimed at investigating the drug preparation of precise powder decoction pieces（PPDP） system, Fallopia multiflora radix preparata（FMRP） was employed in this study. Different specifications of PPDP were prepared, their extract rates were in contrast with the original pieces. Compared the quality uniformity of three batches between FMRP original slices and its PPDP extraction, the similarity of the chemical fingerprints was evaluated, and the contents of common peaks and quality uniformity were compared by relative peak areas. ITS2 sequence was taken as a DNA barcode to identify F. multiflora radix（FMR）. As a result, the extract rate of PPDP was 2.5 times as much as the original slices. The average content of stilbene glucoside from the three original slices and the PPDP extraction were 3.56±2.61 and 13.23±0.37 mg·g-1, respectively; while the RSD were 73.28% and 2.82%. The similarity of the fingerprints of the PPDP extraction was almost the same as that of the original slices, but the content and the uniformity of the common peaks of the PPDP extraction were significantly improved. Thus, FMR was accurately identified using ITS2 sequences. It was concluded that the PPDP considerably improve the decocting rate and quality uniformity, indicating that PPDP could save resources and improve the clinical efficacy.
出处
《世界科学技术-中医药现代化》
CSCD
2017年第1期95-100,共6页
Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology
基金
国家发展改革委
中医药管理局中药标准化项目(ZYBZH-Y-SH-38)子课题:制首乌饮片标准研究
负责人:丘小惠
广东省中医院专项(2015KT1817):岭南中草药DNA条形码分子鉴定和生态适宜性研究
负责人:黄志海
中国中医科学院中医药健康服务专项(ZZ0908067):200种岭南中草药DNA条形码研究
负责人:黄志海
关键词
制何首乌
精准煮散饮片
煎出率
均一性
Fallopia multiflora radix preparata
precise powder decoction pieces
decocting rate
uniformity
