摘要
目的建立一种稳定的小鼠足细胞损伤模型，为进一步研究足细胞的生物特性，以及其相关蛋白与肾性蛋白尿间的关系提供可靠保证。方法应用CCK8检测不同浓度的阿霉素（0．5μg／ml，0．25μg/ml，0．125μg/ml）分别作用24h，48h后，足细胞的抑制率；应用流式细胞术检测测定足细胞凋亡情况;应用R—TPCR检测裂孔膜关键因子nephrin，podocin的表达。结果0．25μg／ml的阿霉素作用24h，抑制率接近50％；流式细胞术及R—TPCR均表明浓度为0．25μ/ml的ADR作用24h后与正常组存在显著性差异。结论0．25μg／ml的阿霉素作用24h建立的足细胞模型稳定可靠，可应用于足细胞研究。
Objective To establish a stable podocyte injury model to further study biological features of podocytes, and the relationship between podocyte-related proteins and renal proteinuria. Methods Podocytes were treated for 24 and 48 h with 0.5, 0.25, 0. 125 μg/mL adriamycin （ADR）, respectively. The inhibition rate of podocytes was detected by using CCK8. Flow cytometry was used to detect the apoptosis of podocyte cells, and R-T PCR to measure the expression of nephrin and podocin. Results The inhibition rate was nearly 50 % in podocytes treated with 0. 25 μg/ mL ADR for 24 h. Flow cytometry and R-T PCR showed that there were significant differences in the apoptosis of podocytes and expression levels of nephrin and podocin between 0. 25μg/mL ADR group and normal group （ P 〈 0. 05 ） . Conclusion The podoeyte injury model induced by 0.25μg/mL ADR is stable and reliable, which can be used for further studies on podocyte injury.
作者
闫梦苗
刘光珍
YAN Mengmiao LIU Guangzhen(Shanxi Institute of Traditional Chinese Medicine, Taiyuan, 030012, China)
出处
《医学分子生物学杂志》
CAS
2017年第1期14-17,共4页
Journal of Medical Molecular Biology
基金
山西省自然科学基金（No.201601D011125）
