摘要
目的观察石斛多糖对慢性疲劳综合征(CFS)大鼠学习记忆能力及血清细胞因子的影响。方法实验大鼠随机分为4组,每组10只:正常组,模型组,石斛多糖低、高剂量组。采用束缚+冷水游泳的复合应激法制备CFS大鼠模型,检测各组大鼠空间学习记忆能力及血清TGF-β和IFN-γ水平。结果石斛多糖可缩短Morris水迷宫测试中大鼠探索路径和搜台潜伏期(P<0.05或P<0.01),提高游泳速度(P<0.05或P<0.01);缩短穿梭箱测试中大鼠潜伏期、进入错误区和电击时间(P<0.05或P<0.01),减少受到电击的次数(P<0.05或P<0.01);并降低大鼠TGF-β水平,升高IFN-γ水平。结论石斛多糖可改善CFS大鼠的认知功能障碍,增强CFS大鼠细胞免疫功能。
Objective：To observe the influence of dendrobium polysaccharides on learning-memory ability and blood serum cytokine of chronic fatigue syndrome mice（CFS） mice. Methods The experimental mice was randomly divided into 4 groups including normal group, model group, low-dose dendrobium polysaccharides group and highdose dendrobium polysaccharides group. The constraint ＋ cold-water swimming stress method was applied to prepare CFS mice model and detect their learning-memory ability as well as serum TGF-β and IFN-γ levels. Results The dendrobium polysaccharides can shorten the exploring path and channel search incubation period in Morris water maze test（P 0.05 or P 0.01） and improve the swimming speed（P 0.05 or P 0.01）;shorten the incubation period, wrong entrance and electric shock time in shutter box test（P 0.05 or P 0.01） and reduce the electric shock times（P 0.05 or P 0.01）; decrease TGF-β level and increase IFN-γ level. Conclusion The dendrobium polysaccharides can improve the cognition impairment and strength cellular immune function of CFS mice.
出处
《长春中医药大学学报》
2017年第1期25-27,共3页
Journal of Changchun University of Chinese Medicine
关键词
石斛多糖
慢性疲劳综合征
大鼠
学习记忆
细胞因子
dendrobium polysaccharides
chronic fatigue syndrome（CFS）
mice
learning-memory
cytokine
