摘要
目的了解西宁地区疑似自身免疫性疾病的老年患者血清抗核抗体(ANA)和可提取核抗原(ENA)抗体的阳性率和阳性分布情况。方法对1 180名老年患者采用间接免疫荧光法检测血清ANA,采用免疫斑点印迹法检测抗ENA抗体。结果 1 180名老年患者血清中,ANA阳性率34.66%(409/1180),以年龄在65至75岁间的ANA阳性为主,阳性率71.15%(291/409)。ANA荧光图形以均质型47.92%(196/409)和核颗粒型42.05%(172/409)为主,核仁型4.15%(17/409),着丝点型3.17%(13/409),胞浆型2.69%(11/409)。409例ANA阳性者中,ENA谱阳性32.02%(131/409),以抗SSA/52kd抗体和抗SSB/La抗体为主,分别占37.41%(49/131)和29.01%(38/131)。结论疑似自身免疫性疾病的老年患者中,以ANA抗体100~320低效价为主。联合检测ANA和ENA谱能提高自身免疫病的早期诊断,尤其对于老年群体,应同时结合患者临床表现,综合分析,不能仅凭阳性检测结果直接诊断。
Aim： to understand the positive rate and positive distribution of anti-nuclear antibody （ANA） and anti-ENA （extractable nuclear antigen） antibodies in elderly patients with suspected autoimmune diseases in Xining area. Method： Blood serum ANA of 1180 elderly patients was detected by indirect immunofluorescence, and Anti-ENA antibody by immuno-blot. Result： Of 1180 elderly patients, the ANA positive rate was 34.66% （409/1180） mainly from 65 to 75 years old and the positive rate was 71.15% （291/409）. The ANA fluorescence pattern was homogeneous in the range of 47.92 （17/409）, 3.17% （13/409） of the centromere type, 2.69% （11/409） of the cytoplasmic type, and 11.5% （17/409）/409）. Of 409 cases, the positive rate of ENA was 32.02% （131/409） mainly in anti-SSA / 52kd antibody and anti-SSB / La antibody, accounting for 37.41% （49/131）and 29.01%（38/131）. Conclusion： It seems that ANA antibody in elderly patients with auto- immune diseases was mainly from 100 to 320 low titer. Combined detection of ANA and ENA spectrum can enhance the early diagnosis of auto-immune disease, especially for the elderly population, and it should be combined with clinical manifestation of the patients and comprehensive analysis, but not just be directly diagnosed with positive test results.
作者
王琰
WANG Yan(Qinghai Red Cross Hospital, Xining Qinghai 810000, China)
出处
《襄阳职业技术学院学报》
2017年第1期91-93,共3页
Journal of Xiangyang Polytechnic
关键词
自身免疫性疾病
老年患者
抗核抗体
可提取核抗原
Auto-immune diseases
elderly patients
anti-nuclear antibody
extracted nuclear antigen
