摘要
目的探讨高糖状态下有氧运动对小鼠心肌组织的保护作用与心肌内miR-222表达的相关性。方法将C57BL/6小鼠分为正常非运动组（SC）、正常运动组（EC）、糖尿病非运动组（SD）和糖尿病运动组（ED）。糖尿病模型建立成功后，EC、ED进行为期5周的游泳训练。实验结束时，光镜下观察小鼠心脏病理学改变，RT-PCR检测心肌内miR-222表达，Western印迹法测定心肌组织中磷酸酶张力蛋白同源物（PTEN）、磷脂酰肌醇-3激酶（PI3K）及蛋白激酶B（Akt）的表达水平。结果（1）光镜下可见糖尿病小鼠心肌结构发生明显改变，细胞排列紊乱，运动干预后病理改变得以改善；（2）无论是正常小鼠还是糖尿病小鼠，运动后心肌组织中miR-222表达均增加（均P〈0.05）；（3）与SC组比较，糖尿病小鼠心肌PTEN表达增强（P〈0.05），PI3K/Akt表达受抑制（均P〈0.05）；运动干预后，糖尿病小鼠心肌内PTEN表达显著降低（P〈0.05），PI3K/Akt通路得以重新激活（均P〈0.05）。结论运动干预可能通过诱导心肌组织miR-222的表达，激活PI3K/Akt信号通路保护高糖状态下的心肌细胞。
Objective To investigate the protective effects of aerobic exercise and miR-222 expression in myocardium of diabetic mice.Methods C57BL/6 mice were divided into 4 groups： normal non-exercise group（SC）, normal exercise group（EC）, non-exercise diabetic group（SD）, and exercise diabetic group（ED）. After the diabetic model was established successfully, EC and ED underwent a swimming training for 5 weeks. By the end of the experiment, light microscope was used to observe the pathological changes of heart, RT-PCR for myocardial miR-222 expression, and Western blot for phosphatase and tensin homolog deleted on chromosome ten（PTEN）, phosphatidylinositol 3-kinase（PI3K）, and protein kinase B（Akt）protein expressions in myocardial tissue.Results （1）Under the light microscope, the diabetic mice had a significant change in myocardial structure, with great disorder in the cell arrangement. After exercise intervention, the lesion was alleviated.（2）MiR-222 expression was increased in the myocardium of normal mice and DM mice after exercise（all P〈0.05）; （3）Compared with SC group, PTEN expression was increased and PI3K/Akt expressions were inhibited in myocardium of diabetic mice（all P〈0.05）. After exercise intervention, the expression of PTEN reduced（P〈0.05）and PI3K/Akt pathway was reactivated in myocardium of diabetic mice（all P〈0.05）.Conclusion Exercise intervention may protect the myocardium under high glucose via inducing miR-222 and activating PI3K/Akt signaling pathway.
出处
《中华内分泌代谢杂志》
CAS
CSCD
北大核心
2017年第1期56-61,共6页
Chinese Journal of Endocrinology and Metabolism
基金
湖北省自然科学基金（2012FFB06807）
关键词
运动
MIR-222
糖尿病
心肌保护
Exercise
miR-222
Diabetes mellitus
Myocardial protection
