摘要
目的建立HPLC法测定咳喘宁合剂中微量组分盐酸苯海拉明的含量。方法色谱柱:Agilent XDB-CN(4.6mm×250mm,5μm);流动相:磷酸盐缓冲液-乙腈(50∶50);检测波长:258nm;流速为1.0ml·min^(-1);进样量为10μl。结果盐酸苯海拉明在20.26~506.5μg·ml^(-1)范围内具有良好的线性关系,回归方程为Y=0.8828X-0.8664(r=1.000);平均回收率为101.4%,RSD值为1.52%。结论该方法具有良好的准确度、精密度和专属性,能较好的控制咳喘宁合剂的质量。
Objective To establish an HPLC method for determination of trace component diphenhydramine hydrochloride in the Kechuanning mixture.Methods Agilent XDB-CN（4.6mm×250mm,5μm）was used.The mobile phase consisted of phosphate buffer-acetonitrile（50∶50）,the detection wavelength was set at 258 nm,the flow rate was 1.0ml·min^（-1）,and the injection volume was 10μl.Results A good linear relationship was observed of diphenhydramine hydrochloride in the range of 20.26-506.5μg·ml^（-1） with a linear equation Y =0.8828 X-0.8664（r =1.000）.The average recovery was 101.4% with an RSD of 1.52%.Conclusion This method can ensure good accuracy,precision and specialization.It can be used for quality coutrol of the Kechuanning mixture.
作者
冯倩倩
陈飞
李翔
刘皈阳
FENG Qian-qian CHEN Fei LI Xiang LIU Gui-yang(Department of Pharmacy,First Affiliated Hospital of Chinese PLA General Hospital,Beijing 100048,China)
出处
《解放军药学学报》
CAS
CSCD
2016年第6期535-537,共3页
Pharmaceutical Journal of Chinese People's Liberation Army
基金
基金项目:<中国人民解放军医疗机构制剂规范>中紫外
高效液相色谱法含量测定系统研究
No.13ZJZ20
关键词
咳喘宁合剂
微量组分
盐酸苯海拉明
HPLC
含量测定
Kechuanning mixture
trace component
diphenhydramine hydrochloride
HPLC
determination
