摘要
目的建立气相色谱法测定口腔抑菌液中麝香草酚、薄荷脑、水杨酸甲酯、桉叶油素4种挥发性成分的含量。方法采用安捷伦CP-1301毛细管柱(30 m×0.32 mm×0.25μm),载气为高纯氮气(99.999%),进样口和检测器(FID)温度为250℃,分流比30∶1,载气流速1.0 mL·min^(-1)。采用程序升温,起始温度为80℃,保持1 min,再以10℃·min^(-1)的速率升温至220℃,保持5 min。结果麝香草酚、薄荷脑、水杨酸甲酯和桉叶油素的线性范围分别为0.0658~0.3289 mg·mL^(-1)(r=0.9978),0.0437~0.2185 mg·mL^(-1)(r=0.9979),0.0582~0.2910 mg·mL^(-1)(r=0.9979)和0.1065~0.5323 mg·mL^(-1)(r=0.9976);4种挥发性成分的回收率均在98%~104%,RSD均小于3.0%。结论该方法操作简便,准确,重复性好,可用于该口腔抑菌液中4种挥发性成分的同时测定。
Objective To establish a gas chromatography to simultaneously determine thymol,menthol,methyl salicylate,and eucalyptol in oral antibacterial solution.Methods Agilent CP-1301(30 m×0.32 mm×0.25μm)was used as the capillary column.Nitrogen(99.999%)was used as the carrier gas,and FID as the detector.The inlet temperature and FID detector temperature was 250℃.The split ratio was 30∶1 and the flow rate was 1.0 mL·min^(-1).The column temperature program was initially 80℃(holding for 1 min)and then increased at the rate of 10℃·min^(-1)to 220℃(holding for 5 min).Results The linearity of thymol,menthol,methyl salicylate,and eucalyptol were 0.0658~0.3289 mg·mL^(-1)(r=0.9978),0.0437~0.2185 mg·mL^(-1)(r=0.9979),0.0582~0.2910 mg·mL^(-1)(r=0.9979)and 0.1065~0.5323 mg·mL^(-1)(r=0.9976).The recoveries were between 98%and 104%,with the RSDs all less than 3.0%.Conclusion The method is simple,accurate,and reproducible,which can simultaneously determine 4 volatile components in oral antibacterial solution.
作者
李笑琳
张佳瑞
张雨萌
赵旻
赵春杰
LI Xiao-lin;ZHANG Jia-rui;ZHANG Yu-meng;ZHAO Min;ZHAO Chun-jie(School of Pharmacy,Shenyang Pharmaceutical University,Shenyang 110016;Zhuhai Essex Biopharmaceutical Company Limited,Zhuhai Guangdong 519000)
出处
《中南药学》
2025年第2期545-548,共4页
Central South Pharmacy
关键词
口腔抑菌液
气相色谱
麝香草酚
薄荷脑
水杨酸甲酯
桉叶油素
含量测定
oral antibacterial solution
gas chromatography
thymol
menthol
methyl salicylate
eucalyptol
content determination
