摘要
目的:探讨子痫前期(PE)患者母血及胎盘组织中HtrA4和syncytin-1的含量变化及其与新生儿出生体重及脐动脉血流动力学参数的相关性。方法:选取2014年2月至2015年5月于河北医科大学第二医院住院分娩的重度PE患者74例(研究组),其中早发组39例、晚发组35例,同期随机选取正常对照组33例。应用彩色多普勒超声检测脐动脉S/D、PI及RI值。采用ELISA法和免疫组化法测定母血和胎盘中HtrA4和syncytin-1含量变化。结果:早发组母血和胎盘组织中HtrA4表达水平显著高于晚发组,晚发组高于对照组(均P<0.05)。早发组的Syncytin-1表达水平明显低于晚发组,晚发组低于对照组(均P<0.05);早发组的S/D值显著高于晚发组和对照组(P均<0.05)。早发组的PI值及RI值显著高于晚发组,晚发组高于对照组,差异有统计学意义(P均<0.05)。结论:HtrA4和Syncytin-1参与PE发病,且与疾病的严重程度相关。PE孕妇母血和胎盘组织中HtrA4水平与新生儿出生体重、脐动脉S/D值、PI值、RI值均呈正相关,而Syncytin-1水平与新生儿出生体重、脐动脉上述指标均呈负相关。
Objective：To evaluate the expression of HtrA4 and Syncytin-1 in maternal plasma and placental tissues,detect the value of S / D,PI,RI in umbilical artery blood flow.To explore the correlation between HtrA4 and Syncytin-1 expression with the pathogenesis of preeclampsia.Methods：Severe preeclampsia and normal pregnant women who were admitted to the Department of Obstetrics and Gynecology,the Second Hospital of Hebei Medical University during Feb.2014 and May 2015.A total of 107 pregnant women were enrolled in the study,with 39 in early onset preeclampsia（ E-PE）,35 in late onset severe preeclampsia（ LS-PE） and 33 in the control group.All women delivered by cesarean section（ CS） were included as cases.S / D,PI and RI were measured by using color doppler ultrasonic.Enzyme-linked immunosorbent assay（ ELISA） was used to measure the concentration of HtrA4 and Syncytin-1 in blood.The distribution of HtrA4,Syncytin-1 in the placenta was observed by Immunohistochemical techniques（ IHC） and mean optical density value（ MOD）.Results：The concentration of HtrA4 in the maternal plasma and placenta tissue was significantly higher in E-PE compared with LS-PE and normotensive pregnancy respectively（ P 0.01,respectively）.On the contrary,the concentration of Syncytin-1 in the maternal plasma and placenta tissue was decreased significantly in E-PE compared to LS-PE and normotensive pregnancy respectively（ P0.01,respectively）.Compared with the control group,the value of S / D in E-PE was significantly higher than the normotensive pregnancy and LS-PE（ P0.05,respectively）.The value of PI,RI were significantly higher than the normotensive pregnancy and LS-PE（ P0.05,respectively）,and as the same with the value of PI,RI in LS-PE was also significantly higher compared with the control group（ P 0.05）.Conclusions：HtrA4 and Syncytin-1 may involved in the pathogenesis of PE,and correlated with severity of PE.There is a negative correlation between the level of HtrA4 and the birth weight of newborns both in maternal plasma and placenta tissue in preeclampsia,and positive correlation between HtrA4 levels and S / D、PI、RI.Whatever a negative correlation between the level of Syncytin-1 and the birth weight of newborns,negative correlation between Syncytin-1 levels and S /D,PI,RI.
出处
《现代妇产科进展》
CSCD
北大核心
2017年第1期5-10,共6页
Progress in Obstetrics and Gynecology
