摘要
[目的]优化超声波法提取凹叶厚朴内生真菌代谢产物的工艺。[方法]采用正交试验设计,以超声温度、超声时间、超声功率为考察因素,以厚朴酚的含量为指标,采用高效液相色谱法,确定发酵液的最佳超声提取条件。[结果]最佳超声提取条件为超声温度50℃、超声时间60 min、超声功率100 k Hz。[结论]该方法能够用于内生真菌发酵液中代谢产物提取。
[Objective] The aim was to optimize the technique for extracting endogenous fungal metabolites from Magnolia officinalis with ultra- sonic method. [ Method ] Using orthogonal test design, with ultrasonic temperature, ultrasonic time, ultrasonic power as investigated factors, the content of magnolol as indicator, adopting HPLC method, the optimum ultrasonic extraction conditions of mycelia were obtained. [ Result ] The optimum ultrasonic extraction conditions were as following： ultrasonic temperature 50℃, ultrasonic time 60 min, ultrasonic power 100 kHz. [ Conclusion] The method can be used to extract endogenous fungal metabolites in the fermented liquid.
作者
耿直
GENG Zhi(Shaanxi Institute of International Trade Commerce, Xi＇an, Shaanxi 712046)
出处
《安徽农业科学》
CAS
2017年第1期122-123,共2页
Journal of Anhui Agricultural Sciences
基金
陕西省教育厅2014年科学研究计划(专项项目
14JK2010)
关键词
凹叶厚朴
内生真菌
超声提取
正交试验
Magnolia officinalis
Endophytic fungi
Ultrasonic extraction
Orthogonal test
