摘要
目的:探讨Wnt7b/β-catenin信号通路在哮喘气道重塑中的作用。方法:选取无特定病原体(SPF)级健康雄性SD大鼠16只,随机分成哮喘组和对照组。哮喘组大鼠用鸡卵清白蛋白(OVA)作为致敏物建立哮喘气道重塑模型,对照组大鼠采用0.9%氯化钠溶液进行处理。肺组织标本予HE染色,观察大鼠肺组织的病理结构,图像分析技术测定大鼠支气管管壁厚度(Wat)和支气管平滑肌厚度(Wam),免疫组织化学法检测肺组织中Wnt7b、β-catenin和c-Myc蛋白的分布和表达,实时荧光定量反转录多聚酶链反应法(q RT-PCR)测定Wnt7b、β-catenin、c-Myc m RNA的表达。结果:哮喘组大鼠Wat和Wam均高于对照组。免疫组织化学法检测显示,哮喘组大鼠肺组织Wnt7b、β-catenin和c-Myc蛋白的表达均高于对照组,差异有统计学意义(P<0.01)。q RT-PCR法检测结果显示,哮喘组大鼠肺组织Wnt7b、β-catenin、c-Myc m RNA的表达均高于对照组,差异有统计学意义(P<0.01)。肺组织中Wat、Wam与Wnt7b、β-catenin、c-Myc的蛋白表达量成正相关。结论:Wnt7b、β-catenin和c-Myc参与哮喘气道重塑的形成。
Objective： To explore the role of Wnt7b/β-catenin signaling pathway in airway remodeling in asthma. Methods： This study enrolled 16 8-week-old healthy male Sprague-Dawley（SD） rats（SPF grade）, weighting from 180-200 g, without specific pathogens, and assigned them into asthma group and normal control group. Asthmatic airway remodeling models were established in rats with ovalbumin while the normal control group was given spray of physiological saline. HE staining was used to observe pulmonary pathological structure of two groups of rats, and color medical image analysis was used to determine the thickness of bronchial wall（Wat） and the thickness of smooth muscles（Wam）. Immunohistochemical method was used to detect the distribution and protein expression of Wnt7 b, β-catenin and c-Myc in the lung tissues, q RT-PCR was used to determine the m RNA expressions of Wnt7 b, β-catenin and c-Myc. Results： Wat and Wam of asthma group were significant higher than those in normal group. The protein expression of Wnt7 b, β-catenin, c-Myc detected by IHC in asthma group were significant higher than those in normal group（P〈0.01, respectively）. The m RNA expression of Wnt7 b, β-catenin, c-Myc detected by q RT-PCR in asthma group were significant higher than those in normal group（P〈0.01, respectively）. There were significantly positive correlation between Wat and the protein expression of Wnt7 b, β-catenin and c-Myc, also between Wam and the protein expression of Wnt7 b, β-catenin and cMyc. Conclusion： Wnt7 b, β-catenin and c-Myc play important roles in airway remodeling in asthma.
出处
《温州医科大学学报》
CAS
2017年第1期14-18,共5页
Journal of Wenzhou Medical University
基金
浙江省自然科学基金资助项目(LY15H010006)
温州市科技计划项目(Y20130234)
浙江省科技厅科研基金资助项目(2016C33182)
浙江省卫生高层次创新人才项目
