摘要
研究腺苷等8种物质对Cordyceps militaris FFCC 5111液体发酵生产虫草素的影响,并考察了其发酵动力学特征。结果表明,添加腺苷、苯丙氨酸、腺嘌呤、甘氨酸和L-谷氨酰胺均可提高虫草素产量,其中添加腺苷1.5g/L的发酵液中虫草素产量最大,为772.5mg/L,是对照组的9.39倍。在50L发酵罐中,添加1.5g/L腺苷的麦芽汁培养基进行分批发酵,建立动力学模型,其拟合值与实验值的相对误差小于8%,能良好地反映C.militaris FFCC 5111在补加腺苷的麦芽汁培养基中的发酵动力学特征。
The effects of different additives on cordycepin production by submerged culture of Cordyceps militaris FFCC 5111 were investigated.The result indicated that the additives of adenosine,phenylalanine,adenine,glycine and L-glutamine could result in a large improvement in cordycepin production by submerged fermentation of C.militaris,especially adding 1.5 g/L adenosine,which could result in the highest cordycepin production of 772.5 mg/L,exhibiting a9.39-fold increase than that obtained in the basal medium culture.Adding 1.5g/L adenosine as a medium in a 50 Lbatch system,the kinetic model for C.militaris FFCC 5111 was based on the Logistic and Luedeking-Piret equations for cell growth and product formation.The relative error between the experimental value and fitted value was less than 8%,indicating that the model could reflect the kinetics feature of batch fermentation of C.militaris FFCC 5111 in maltose medium supplemented with adenosine.
作者
闫瑞
周广麒
YAN Rui ZHOU Guangqi(School of Biological Engineering, Dalian Polytechnic University, Dalian 116034, China)
出处
《大连工业大学学报》
CAS
北大核心
2016年第5期321-324,共4页
Journal of Dalian Polytechnic University
基金
辽宁省高校创新团队支持计划项目(LN2012011)
关键词
蛹虫草
虫草素
腺苷
动力学
Cordycep militaris
cordycepin
adenosine
kinetics
