摘要
目的:研究血竭生肌膏对糖尿病模型大鼠干预作用,研究其促进糖尿病创面愈合的机制。方法:应用链脲佐菌素腹腔注射法制作糖尿病大鼠模型,通过手术损伤和50%冰醋酸刺激法制作糖尿病性溃疡模型。将大鼠随机分为假手术组、糖尿病性溃疡模型组、中药治疗组、西药对照组,分别给予不同的干预措施,通过免疫组织化学方法检测治疗前后创面基质金属蛋白酶(MMPs)及其抑制物(TIMP)变化。结果:MMP-2在假手术组、模型组、西药组、中药组的表达分别为(0.068 17±0.021198)、(0.139 33±0.012 094)、(0.081 50±0.011 203)、(0.078 83±0.012 073);而MMP-9的表达分别为(0.074 83±0.006 969)、(0.13850±0.028 452)、(0.081 50±0.011 962)、(0.074 83±0.009 663)。假手术组大鼠溃疡创面MMP-2、MMP-9的表达明显低于其它3组(P<0.01);模型组大鼠溃疡创面MMP-2、MMP-9的表达明显高于西药组、中药组(P<0.01),中药组大鼠溃疡创面MMP-2、MMP-9的表达明显低于西药组(P<0.01)。TIMP-1在假手术组、模型组、西药组、中药组的表达分别为(0.081 33±0.013 307)、(0.069 67±0.008 524)、(0.079 17±0.011 974)、(0.132 50±0.011 415)。假手术组大鼠溃疡创面TIMP-1的表达明显高于模型组、西药组、中药组(P<0.01),模型组大鼠溃疡创面TIMP-1的表达明显低于西药组和中药组(P<0.01),中药组大鼠溃疡创面TIMP-1的表达明显高于西药组(P<0.01)。结论:血竭生肌膏通过抑制糖尿病大鼠溃疡创面MMP-2、MMP-9的表达,提高TIMP-1的表达,从而促进糖尿病溃疡创面的愈合。
Objective To study the effect of XueJieShengJi ointment on matrix metalloproteinases expres- sion in diabetic rats. Methods Diabetic rat model was made by intraperitoneal injection of streptozotocin, and the diabetic ulcer model was induced by surgical injury and 50% glacial acetic acid stimulation. The rats were randomly divided into sham operation group, diabetic ulcer model group, chinese medicine treatment group and western medicine group（control group）, and were given different interventions. Wound matrix metalloproteinases changes were assessed by immunohistochemical method before and after the treatment. Results The expres- sion of MMP-2 in sham operation group, model group, control group, Chinese medicine group were （0.068 17 ± 0.021 198）, （0.139 33 ± 0.012 094）, （0.081 50 ± 0.011 203）, （0.078 83 ± 0.012 073）, respectively. The expres- sion of MMP-9 in sham operation group, model group, control group, Chinese medicine group were （0.074 83 ± 0.006 969）, （0.138 50 ± 0.028 452）, （0.081 50 ± 0.011 962）, （0.074 83 ± 0.009 663）, respectively. The expres- sion of MMP-9 and MMP-2 in the sham operation group was significantly lower than that of model group, con- trol group, and Chinese medicine group （P 〈 0.01）, The expression of MMP-9 and MMP-2 in ulcers of modelgroup rats was significantly higher than that of con- trol group and Chinese medicine group （P 〈 0.01）. In Chinese medicine group, MMP-2 and MMP-9 ex- pression was significantly lower than in control group （P 〈 0.01）. TIMP-1 in sham operation group, model group, control group, traditional Chinese medicine （0.079 17± 0.011 974）, （0.132 50± 0.011 415）, group was significantly higher than in the model group were （0.081 33 ± 0.013 307）, （0.069 67 ± 0.008 524）, respectively. The expression of TIMP-1 in the sham operation group, control group and Chinese medicine group （P 〈 0.01）. The expression of TIMP-1 in the model group was significantly lower than in control group and Chinese medi- cine group （P 〈 0.01）. In Chinese medicine group, TIMP-1 expression was significantly higher than in control group （P 〈 0.01）. Conclusion XueJieShengJi ointment improves ulcer recovery in diabetic rats by inhibiting the expression of MMP-2 and MMP-9 and increasing the expression of TIMP-1.
出处
《中国中西医结合外科杂志》
CAS
2016年第6期571-575,共5页
Chinese Journal of Surgery of Integrated Traditional and Western Medicine
基金
北京中医药大学2015年度基本科研业务费中青年教师项目(2015-JYB-JSMS073)
关键词
血竭生肌膏
糖尿病
大鼠
基质金属蛋白酶
XueJieShengJi ointment
diabetic rats
matrix metalloproteinases
