摘要
目的:探讨不同吸氧时间高压氧(HBO)治疗对脑出血(ICH)大鼠血肿周围水通道蛋白(AQP4)和超氧化物歧化酶(SOD)表达的影响,从而探讨HBO治疗脑出血的最佳吸氧时间。方法:正常组Wistar大鼠5只,胶原酶诱导法建立Wistar大鼠脑出血(ICH)模型125只,随机分为对照组(不用高压氧干预,25只)和实验组(ICH后6小时给予高压氧干预,100只),实验组根据吸氧时间不同又分为ICH+HBO 40min组,ICH+HBO 60min组,ICH+HBO 80min组,ICH+HBO 100min组,共4个亚组,各25只。每组大鼠分别在行HBO治疗第1、3、5、7、14天后于相同时间点处死,之后采用干湿比重法测定出血灶周围脑组织含水量,用免疫组化法检测血肿周边脑组织中AQP4蛋白表达情况,黄嘌呤氧化酶法测定SOD活力。结果:(1)出血灶周围脑水含量:实验组较对照组相比,各时间点脑组织水含量均减少且有差异(P<0.05)。(2)大鼠脑组织AQP4表达的变化:对照组大鼠的AQP4表达在ICH术后第1天开始升高,第3天持续升高,第5天达到高峰,之后逐渐下降直至第14天。HBO实验组各时间点AQP4的表达较对照组减少,差异有显著性意义(P<0.05)。其中ICH+HBO 60min组第7天下降的幅度最大,较其他三组相比具有显著性意义(P<0.01)。(3)大鼠脑组织SOD表达的变化:对照组和实验组SOD上调表达规律与AQP下调表达一致,其中ICH+HBO 60min组回升幅度最大,其在第14天表达的值与其他三组相比均有显著性差异(P<0.01)。结论:HBO可以减轻脑水肿,其中最佳吸氧时间是60min。其机制可能与降低了出血灶周围AQP4表达水平、提高了SOD表达水平有关。
Objective： To study the effects of different oxygen uptake time of hyperbaric oxygen on perihematomal ede- ma, expression of aquaporin-4 and superoxide dismutase in rats with intracerebral hemorrhage, for exploring the best oxygen uptake time of hyperbaric oxygen. Method： 125 model rats were established by collagenase-induced cerebral hemorrhage and 5 Wistar rats served as normal control. 125 ICH rats were randomly divided into the control group （without intervention with hyper- baric oxygen after brain hemorrhage, n=25） and the experimental group （intervention with hyperbaric oxygen af- ter brain hemorrhage, n=100） （divided into ICH＋HBO 40min group, ICH＋HBO 60rain group, ICH＋HBO 80min group and ICH＋HBO 100min group based on different oxygen uptake time, n=25 for each group ）. After 1 day, 3 days, 5 days, 7 days or 14 days HBO therapy, rats in each group were executed at the same time point . The drying wet method was used to determine the moisture of brain tissue around the hemorrhagic fo- cus. The immunohistochemistry and xanthine oxidase technique were used to detect the expression of AQP4 and SOD vitality, respectively. Result： The water content of brain tissue around the hemorrhagic focus： The experimental groups has the less water content of brain tissue when compared with the control groups at each time point （P〈0.05）.The expression of AQP4： In the control, the expression of AQP4 increased at the 1st day after ICH and reached the peak at the 5th day after ICH. After that, the expression of AQP4 presented decreased trend. At each time point, the HBO experimental groups showed less expression of AQP4 compared with the control groups （P〈 0.05）. There was the largest decline at the 7th day for the ICH＋HBO 60min group, which produced the statisti- cally significant difference compared with the other three groups（P〈 0.01）.SOD content： For the control and experimental groups, the SOD showed the increased trend that was consistent with the decreased trend of the AQP4. The ICH＋HBO 60min group has the largest rise and shows significant difference at the 14th day when compared with the other three groups （P〈 0.01）. Conclusion： HBO can reduce perihematomal edema and the best oxygenuptake time is 60 mins. It may be re- lated to the decreased AQP4 expression and the increased SOD level.
出处
《中国康复医学杂志》
CAS
CSCD
北大核心
2016年第11期1213-1218,共6页
Chinese Journal of Rehabilitation Medicine
关键词
高压氧
吸氧时间
脑出血
脑水肿
水通道蛋白-4
超氧化物歧化酶
hyperbaric oxygen
oxygen uptake time
intracerebral hemorrhage
brain edema
aquaporin-4
super-oxide dismutase
