摘要
从大豆植株中分离到一株具有广谱抗菌活性的拮抗细菌,命名为XZ-2。抑菌活性测定显示XZ-2对甘薯黑斑病菌、大蒜叶枯病菌等8种植物病原真菌均具有较强的抑菌活性,抑菌直径均在20 mm以上。采用分光光度法绘制了XZ-2的生长曲线,结果显示该菌株在LB液体摇培12 h时基本达到对数生长期,该生长曲线的绘制为后期XZ-2的活性物质发酵条件优化奠定了基础。通过PCR扩增16Sr DNA片段并采用MEGA 6.0构建系统发育进化树,初步将该菌株鉴定为多粘类芽孢杆菌。
In this experiment, an endophytic bacteria XZ-2 with broad spectrum antimicrobial activity was isolated from soybean plant. Antibiotic activity assay showed that XZ-2 had strong antibiotic ability against 8 phytopathogenic fungi, and the dimeters of inhibition zone were larger than 20 ram. The growth curves of XZ-2 were drawn by spectrophotometric method, the result showed shaking XZ-2 in liquid LB medium for 12 h could basically reach the logarithmic growth phase, the growth curve drawing for XZ-2 may be useful for fermentation conditions optimization in further study. 16SrDNA fragment was amplified by PCR and the phylogenetic tree was constructed by software MEGA 6. 0, and then XZ-2 was identified as PaenibaciUus polymyxa. This study showed that XZ-2 had brilliant biological control application prospect.
作者
王波
周涧楠
黄忠勤
王幸
丁震乾
常勇
周兴根
WANG Bo ZHOU Jian-nan HUANG Zhong-qin WANG Xing DING Zhen-qian CHANG Yong ZHOU Xing-gen(Xuzhou Academy of Agricultural Sciences, Xuzhou 221131, China)
出处
《大豆科学》
CAS
CSCD
北大核心
2016年第6期997-1001,共5页
Soybean Science
基金
国家现代农业产业技术体系建设专项(CARS-04-CES22)
优质多抗超高产麦稻新品种徐麦31
徐68优201产业化(BA2014074)
关键词
多粘类芽孢杆菌
拮抗活性
大豆
Paenibacillus polymyxa
Antibiotic activity
Soybean
