摘要
在基质辅助激光解吸电离飞行时间质谱 (MALDI TOF MS)和电喷雾质谱 (ESI MS)测定蛋白质分子分子量的过程中 ,适当提高样品的酸度 ,可提高分析测试的灵敏度。在选定最佳样品分子浓度的基础上 ,通过适当加入三氟乙酸 (TFA)来调整测试样品的酸度 ,准确测定了标准蛋白质 溶菌酶 (lysozyme)的分子量 ,并且对蛋白质分子在“软电离”质谱中 。
The acid effects of some proteins on measuring their molecular weights were studied using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry ( MALDI-TOF-MS) and electrospray ionization mass spectrometry (ESI-MS). It was found that the signal intensity was enhanced through adjusting the acid concentration in some protein samples. In this paper, both MALDI-MS and ESI-MS was applied to examine the molecular weights of several standard proteins. And the proper acid concentration was detected in these spectra. In the meanwhile, it demonstrates that some associations of proteins in solution can be preserved into the gas phase and observed by the 'soft ionization' mass spectrometry.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2002年第8期938-941,共4页
Chinese Journal of Analytical Chemistry
关键词
蛋白质
分子量测定
酸效应
基质辅助激光解吸电离飞行时间质谱
电喷雾质谱
溶菌酶
matrix assisted laser desorption/ionization-time-of-flight mass spectromery
electrospray ionization mass spectrometry
bovine serum albumin
lysozyme
trypsinogen
