摘要
将偏振技术、同步技术与三维技术结合起来的三维同步偏振荧光光谱(TDSPS)能分辨蛋白质溶液中的色氨酸(Trp)和酪氨酸(Tyr)残基,具有同步光谱分辨率高、三维技术信息丰富的优点.本文用TDSPS表征牛血清白蛋白(BSA)、人血清白蛋白(HSA)受各种因素影响(酸效应、碱效应、盐效应、猝灭剂等)时Trp, Tyr残基荧光光谱的变化,用于区分HSA和BSA.
Based on polarizing, synchronous and three-dimensional technologies, a new three-dimensional-synchronous-polarized spectrofluorimetry (TDSPS) was used to distinguish tryptophan (Trp) and tyrosine (Tyr) residues in albumin solution with the advantages of abundant information of three-dimensional technology and high resolution of synchronous spectrofluorimetry. In this paper, the fluorescent spectral changes of Tyr and Trp residues in bovine serum albumin (BSA) and human serum albumin (HSA) were studied by obtaining the characteristic information that had been influenced by various factors (acidic, basic, salt and quenching reagents effects, etc.) with TDSPS. As a result, HSA and BSA could be distinguished.
出处
《化学学报》
SCIE
CAS
CSCD
北大核心
Acta Chimica Sinica
关键词
三维同步偏振荧光光谱
蛋白质
发光行为
色氨酸
酪氨酸
牛血清白蛋白
人血清白蛋白
three-dimensional synchronous-polarized spectrofluorimetry ( TDSPS)
tryptophan (Trp)
tyrosine (Tyr)
bovine serum albuntin (BSA)
human serum albumin (HSA)
