摘要
采用有机合成法合成茶多酚-锗化合物.运用反相液相色谱技术初步分离茶多酚和茶多酚锗的酚类组成.选用基质辅助激光解吸电离化飞行时间(MALDI-TOF)质谱技术分析茶多酚及茶多酚-锗的组成和结构.结果表明,茶多酚主要由儿茶素及酚类化合物组成,其中(-)表没食子儿茶素没食子酸酯(EGCG)、(-)表没食子儿茶素没食子棓酸甲酯(EGC-MG)和表三羟基黄烷-3-醇没食子酸酯(EZG)易与Ge4+反应且形成含锗的酚类化合物.茶多酚及茶多酚-锗化合物均有抑制肺癌细胞生长的能力,其抑制速率随着酚类化合物浓度增加而提高,但茶多酚锗抑制速率高于茶多酚近1倍.
The compound of tea polyphenol-germanium （TP-Ge^4＋） was synthesized by typical technology of organic synthesis. The polyphenol compositions of tea polyphenol and tea polyphenol-germanium were effectively separated by RE-HPLC techniques. Matrix-assisted laser desorption ionization/time of flight （MALDI-TOF） mass spectrometry was selected to analyze the composition, structure and stability of tea polyphenol （TP） and TP-Ge^4＋. The results showed that TP was mainly composed of catechin and polyphenolic compounds. These polyphenolic compounds such as epigallo-catechin gallate （EGCG）, epigallo-catechinmethyl gallate （EGC- MG）,epiafzelechin gallate （EZG） reacted easily with germanium oxide to consistute a series compounds of the polyphenolic-germaniurn. In addition,both TP and TP-Ge^4＋ showed capacities for inhibitting growth of lung-cancer cells. This inhibiting rate would enhance with the concentration increment both compounds. However, the inhibiting rate with TP-Ge^4＋ showed higher approximately one multiple than that of TP.
出处
《厦门大学学报（自然科学版）》
CAS
CSCD
北大核心
2007年第2期258-261,共4页
Journal of Xiamen University：Natural Science
基金
国家自然科学基金(30470372)
三明市科技项目(2004-L-1)
福建省教育厅科技项目(JB03238)资助
关键词
茶多酚
茶多酚锗
组成
分子结构
肺癌细胞
tea polyphenol
tea polyphenol-germanium
composition, structure
lung-cancer cell
