摘要
以实验室保存的一株费氏丙酸杆菌CS1420(Propionibacterium freudenreichii CS1420)为试验菌株。首先考察了不同初始pH下对其发酵产细菌素的影响。结果表明:以大肠杆菌ATCC25922为指示菌,当培养基初始pH为6.0时,细菌素抑菌效果最好;以Saccharomyces cerevisiae 2-10515为指示菌,当培养基初始pH为5.5时,细菌素抑菌效果最好。然后用丙酸调节细菌素粗提物的pH进行抑菌试验,结果表明,pH对粗提得到的细菌素抑菌性影响很大,当用丙酸将细菌素溶液的pH调至5.5时,细菌素的抑菌活性有显著提高。
Propionibacterium freudenreichii CS1420 was as experimental strains. Firstly,the effects of different pH on fermentation of Propionibacterium freudenreichii Bacteriocins were investigated. The result showed：the antimicrobial activity were best by using Eoli ATCC25922 as indicator strains when the medium initial pH was 6.0,the antimicrobial activity were best by using Saccharomyces cerevisiae 2-10515 as indicator strains when the medium initial pH was 5.5. Then,bacteriocins crude extract pH were adjusted and then bacteriostatic exper-iments were carried out. The result showed：the effect of pH on crude extract bacteriocins was tremendous. The antimicrobial activity of bacteriocins improved significantly when crude extract bacteriocins solution pH were adjusted 5.5 with propionic acid.
作者
郑丽雪
郭晨
谢建松
唐亚进
王立梅
齐斌
ZHENG Li-xue GUO Chen XIE Jian-song TANG Ya-jin WANG Li-mei QI Bin(College of Biology and Food Engineering, Changshu Institute of Technology, Changshu 215500, Jiangsu, China Suzhou Key Laboratory of Food and Biotechnology,Changshu 215500,Jiangsu,China)
出处
《食品研究与开发》
CAS
北大核心
2016年第16期175-178,共4页
Food Research and Development
基金
江苏省科技支撑计划项目(BE2013339)
苏州市科技计划项目(SYN201226)
苏州市科技计划项目(SYN201417)
常熟理工学院大学生实践创新训练计划项目
关键词
费氏丙酸杆菌
PH
细菌素
抑菌性
Propionibacteriumfreudenreichii
pH
bacteriocins
antimicrobial activity
