摘要
通过生物信息学分析耐辐射奇球菌DR_2327和DR_2328基因及编码蛋白的基本性质,利用PCR方法克隆DR_2327和DR_2328的全基因,连接至p GEM-T载体上,转化至大肠杆菌JM109中,并进行双酶切鉴定。生物信息学分析结果表明,DR_2327和DR_2328基因位于同一操纵子中,且DR_2328蛋白具有组氨酸激酶活性,DR_2327蛋白具有反应调节子功能,提示两者很可能组成一对双组分系统。体外实验表明,DR_2327和DR_2328基因的外源表达在一定程度上提高了大肠杆菌对紫外辐照和H2O2的耐受能力。
The main characteristics of DR_2327and DR_2328 in Deinococcus radiodurans were analyzeed by bioinformatics. Full-length gene of DR_2327 and DR_2328 was amplified by PCR, and constructed in p GEM-T vector. Transformed recombinant vector into E.coli JM109 and followed by restriction enzyme digestion. DR_2327 and DR_2328 gene was cloned successfully. Bioinformatic analysis results showed that DR_2327 and DR_2328 gene located in the same operon, and DR_2328 protein with histidine kinase activity, DR_2327 protein with response regulator activity. It is suggested that DR_2327 and DR_2328 constitute a two-component system in Deinococcus radiodurans. In vitro experiments showed that DR_2327 and DR_2328 could enhance tolerance to UV radiation and H2O2 of E.coli.
出处
《辐射研究与辐射工艺学报》
CAS
CSCD
2016年第5期25-32,共8页
Journal of Radiation Research and Radiation Processing
