摘要
研究耐辐射球菌PprI蛋白对哺乳动物急性放射损伤的防护作用,初步探讨其作为一种新的抗辐射蛋白的作用机制。将PprI蛋白或生理盐水注射入小鼠肌肉内,观察辐照后第1、7、14、28天小鼠外周血象以及小鼠骨髓、胸腺和脾脏淋巴细胞的凋亡情况。研究结果表明:与生理盐水注射组相比,PprI蛋白注射组外周血白细胞计数于照后第28天显著增高(p<0.05);外周血小板数和淋巴细胞百分率于照后第1、7天显著增高(p<0.05);PprI蛋白注射组脾脏淋巴细胞凋亡率在照后第1、7、14天明显降低(p<0.05);胸腺淋巴细胞凋亡率于照后第14和28天明显降低(p<0.05);骨髓细胞凋亡率在照后第1、14和28天明显降低(p<0.05);并且骨髓细胞凋亡率于照后第28天基本恢复正常。上述结果初步表明,耐辐射球菌PprI蛋白对哺乳动物急性放射损伤有着明显的防护作用。
In this paper the effect of PprI protein of deinococcus radiodurans to protect mice from acute radiation injury had been investigated meanwhile the relative mechanism had also been discussed. After injecting PprI protein or physiological saline into the muscle of mice, the blood cell count and the apoptotic rates of bone marrow cells, spleen cells and thymocyte cells on the 1st, 7th, 14th, and 28th day after irradiation were investigated. Compared with the physiological saline injection group, it has been found that the leucocyte number is significantly higher on the 28th day after irradiation （p〈0.05）, the platelet number and the lymphocyte ratio is obvious higher on the lth day and the 7th day after irradiation （p〈0.05） in peripheral blood of the PprI protein injection group. On the other hand, the apoptotic rate of spleen cells is significantly lower on the lth, 7th, and 14th day （p〈0.05）, the apoptotic rate of thymocytes is significantly lower on the 14th and 28th day （p〈0.05）, the apoptotic rate of bone marrow cells is significantly lower on the 1st, 14th and 28th day （p〈0.05）, and the apoptotic rate of bone marrow cells is return to normal level on the 28th day after irradiation. The result indicates that the PprI protein of deinococcus radiodurans can prevent mice from the acute radiation injure apparently.
出处
《辐射研究与辐射工艺学报》
CAS
CSCD
2012年第5期286-290,共5页
Journal of Radiation Research and Radiation Processing
基金
国家自然科学基金项目(81072236)
国防基础科研项目(A3820060138)
江苏省高校优势学科建设工程资助
关键词
耐辐射球菌
PprI蛋白
放射损伤
防护
Deinococcus radiodurans, Pprl protein, Radiation injury, Prevention and protection
