摘要
目的通过检测再生基因Ⅳ(Regenerating geneⅣ,RegⅣ)和Ki67在溃疡性结肠炎(ulcerative colitis,UC)诱发结肠癌小鼠模型中的表达,探讨RegⅣ对UC诱发结肠癌早期诊断、判断预后的价值。方法 60只BALB/c小鼠分为正常对照组、模型组,采用偶氮氧甲烷(Azoxymethane,AOM)联合葡聚糖硫酸钠(Dextran sulfate sodium,DSS)的方法建立UC癌变的小鼠模型,HE染色观察小鼠结肠组织病理变化,采用免疫组化检测不同时期肠道组织中Ki67和RegⅣ蛋白的表达,实时荧光定量PCR检测此两项指标mRNA的表达。结果癌变组小鼠结肠黏膜中Ki67的表达水平较其他组明显升高(P<0.05),轻-中度不典型增生组RegⅣ的表达水平较癌变组明显升高(P<0.05),免疫组化法统计学结果与RT-PCR一致。结论 Reg IV在肠黏膜癌变过程中表达过量可能先于Ki67,提示RegⅣ可作为预测UC诱发结肠癌的良好分子标志物。
Objective To evaluate the early diagnostic and predictive value of Regenerating gene Ⅳ （Reg Ⅳ ） in ulcerative colitis （UC） cancerization by detecting Reg Ⅳ and Ki67 in UC cancerization mice model. Methods Sixty mice were divided into the experimental group and the control group in the study. UC caneerization mice model was set up byazoxymethane combined with dextran sulfate sodium. Colon tissue pathological changes were observed by HE dyeing. Gene expression was investigated by immunohistochemistry, mRNA expression was investigated by reversetranserip- tion-PCR. Results Ki67 expression in colonic mucosa of colon cancer group mice was obviously higher than other groups （P 〈 0.05 ）. Reg Ⅳ expression in light to moderate dysplasia group was apparently higher than that in the cancer- ous group （P 〈 0.05）. Statistical results of immunohisto-chemical were consistent with RT-PCR. Conclusion The high expression of Reg Ⅳ may precede Ki67 in cancerous process of intestinal mucosal. Reg Ⅳ level is a good cancer molecu- lar marker for predicting the cancerization in UC patients.
出处
《胃肠病学和肝病学杂志》
CAS
2016年第10期1116-1119,共4页
Chinese Journal of Gastroenterology and Hepatology
