摘要
目的通过对1996～1998年采集的艾滋病病毒1型(HIV-1)毒株样本的env基因的序列分析,阐明在中国流行的E亚型HIV-1毒株的特点、来源和传播方式。为中国E亚型HIV-1疫苗的研制和应用提供基础资料。方法 从HIV感染者淋巴细胞(PBMC)中提取前病毒DNA.使用嵌套式聚合酶链反应(PCR)方法扩增HIV-1的env基因的C2V5区。PCR产物不经克隆直接测序并使用GCG软件包进行序列分析。结果样品采自1996～1998年中国29个省(自治区,直辖市),总共发现37个E亚型HIV-1感染者。他们中大部分是通过性途径感染(23人,占62.2％);部分在静脉吸毒人群中发现(10人,占27.0％);少数是在职业献血员中发现(4人,占10.8％)。经C2-V3区序列分析发现,大部分中国E亚型HIV-1毒株与泰国株很相近,而与非洲株相差很大。而来自广西壮族自治区的毒株与越南吸毒人群中的流行株U48720相一致;系统树分析结果发现,中国的E亚型HIV-1株与泰国(CM240X、H93TH966)、越南(U48720)的代表株聚在一起。结论 中国E亚型HIV-1毒株目前仅在东南沿海地区流行,涉及静脉吸毒、输供血和性乱等各种人群,通过env区的序列分析发现其主要来源于泰国,部分来源于与中国接壤的越南。
Objective To investigate the characteristics, origin and transmission pattern of the HIV-1 subtype E infection in China.Methods Blood was collected from HIV- 1 infected individuals in China.The DNA from peripheral blood mononuclear cells was used as template for amplification of C2-V5 region of HIV env gene. The genes were sequenced and analyzed together with epidemiologi-cal data. Results A total of 37 people infected with subtype E HIV-1 were found in China between 19% and 1998.The majority of them(23,62.2%)were infected through sexual contacts; some were through injecting drugs(10,27.0% )and a small number(4,10.8% ) through contaminated blood. The geographic location of the sexually transmitted subtype E cases were mainly in the southwest border and southeast coastal regions.There were two clusters of subtype E HIV- 1 strains on the phylogenetic tree.One group made by the subtype E sequences from Guangxi and Jiangxi provinces clustered with the subtype E sequences from Vietnam(U48720), and the other made by sequences from Guangdong,Shengzhen and Fujian clustered with the subtype E sequences of Thailand(CM240) .All the subtype E sequences from China were quite different from the African subtype E strain(CF40). Discussion It is concluded that the subtype E HIV - 1 is mainly circulating in the group of sexual transmission in China.Those subtype E HIV- 1 strains originated from two main directions, one from Thailand to the south east coastal regions(Guangdong, Shengzhen and Fujian), and the other from Vietnam to the southwest border regions.
出处
《中国性病艾滋病防治》
2002年第4期200-203,共4页
Chinese Journal of Std & Aids Prevention and Control
