摘要
目的探讨富含亮氨酸重复序列免疫球蛋白样蛋白1(Lrig1)在结肠癌组织和细胞株中的表达,并比较其与正常结肠组织和正常结肠上皮细胞表达的差异性。方法采用免疫组化、逆转录聚合酶链式反应(RTPCR)和蛋白印迹(WB)方法检测结肠癌组织和癌旁正常组织以及各细胞株中的表达水平,并比较其差异性。结果免疫组化结果显示,结肠癌组织中Lrig1的表达水平(31.43%,22/70)明显低于癌旁正常结肠组织(86.11%,31/36)。Lrig1在结肠癌组织中的表达与分化、分期及有无淋巴结转移有关(P<0.05),与性别、年龄和肿瘤大小无关(P>0.05)。RT-PCR结果显示,与正常肠上皮细胞株NCM460(0.95±0.12)比较,Lrig1 mRNA在结肠癌细胞株SW480和HCT116中相对表达量降低,分别为(0.65±0.09)和(0.47±0.06),组间比较具有统计学意义(P<0.05)。WB的结果显示,与NCM460(0.98±0.16)比较,Lrig1蛋白在SW480和HCT116细胞中的相对表达量降低,分别为(0.71±0.06)和(0.54±0.05),组间比较差异具有统计学意义(P<0.05)。结论 Lrig1在结肠癌的发生和发展中可能起着抑癌基因的作用。
Objective To detect the expression of leucine-rich repeats and immunoglobulin like domain protein 1 （ Lrig1 ） in human colon cancer tissues and cell lines, normal colon tissue, and colonic epithelium and to compare the expressions in cancer and normal tissures. Methods We collected 70 colon adenocarcinoma tissue samples and 36 normal colon tissues adjacent to the tumors from 70 inpatients with surgery but without radiotherapy or chemotherapy before the operation. We used immunohistochemical method to detect Lrigl expression in colon cancer tissues and adjacent normal tissues ;we also used reverse transcriptase-PCR （RT-PCR） and Western blot to detect Lrigl mRNA and protein in colonic epithelial cells;then the differences in the expressions in different tissues were analyzed. Results The expression of Lrigl in colon cancer tissues （ 31.43 % ,22/70） was obviously lower than that （ 86. 11% ,31/36） in normal tissues adjacent to the tumor. The expression of Lrigl in colon cancer tissues was correlated with the differentiation and staging of tumors and the status of lymph node metastasis （ P 〈 0. 05 for all） but not related to tumor size, gender and age of the patients （P 〉 0. 05 for all）. The results of RT-PCR illustrated that the relative expression of Lrigl mRNA in colon cancer cells （SW480 and HCT116） was significantly lower than in normal colon epithelial cells （NCM460） （0. 65 ± 0. 09 and 0.47±0. 6 vs. 0. 95±0. 12） （both P 〈0. 05） ;the relative expressions of Lrigl protein in the SW480 and HCT116 cells （0. 71 ±0. 06 and 0. 54 ±0. 05） were also significantly lower than that in the NCM460 cells （both P 〈0. 05） based on the results of Western blot test. Conclusion Lrigl may play the role of a cancer suppressor gene in the occurrence and development of colon cancer.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2016年第10期1343-1345,共3页
Chinese Journal of Public Health
关键词
结肠癌
免疫组化
RT-PCR
WB
LRIG1基因
colon cancer
immunohistochemistry
reverse transcriptase-PCR
Western blot
Lrig1 gene
