摘要
目的探讨PI3K、AKT2在胃癌、癌旁组织中的表达,分析其表达水平与临床病理特征和预后的关系。方法用组织芯片和免疫组织化学法检测189例胃癌组织、54例癌旁组织、32例正常胃黏膜中PI3K、AKT2的表达。结果胃癌、癌旁组织和正常胃黏膜PI3K阳性表达率分别是76.7%、25.9%和25.0%。AKT2阳性表达率分别是75.7%、27.8%和37.5%。胃癌组织PI3K表达与有无淋巴结转移、浸润深度、分化程度和Lauren分型有关(P<0.05),与肿瘤大小无关(P>0.05)。AKT2表达与肿瘤大小、有无淋巴结转移、浸润深度、分化程度、Lauren分型有关(P<0.05)。单因素生存分析显示,PI3K、AKT2阳性组对胃癌患者生存期的影响有统计学意义(P<0.05),Cox多元回归分析显示,AKT2的异常表达可以作为影响胃癌预后的独立因素(P<0.05)。结论 PI3K/AKT2细胞信号传导通路参与胃癌的发生、发展、浸润转移,是胃癌发生的晚期事件。AKT2的表达可以作为胃癌预后的独立指标。
Objective To investigate the expression of PI3K and AKT2 in gastric cancer and pericancerous tissue and its relationship with tumor clinicopathological features and survival of patients. Methods The expression of PI3K and AKT2 was examined with tissue microarray and immunohistochemistry in samples of normal gastric mucosa（n=32）,pericancerous tissue（n=54）,and gastric cancer（n=189）. Results The positive rates of PI3K in gastric carcinoma,pericancerous tissue and normal gastric mucosa were 76.7%,25.9% and 25.0%,respectively.The positive rates of AKT2 in gastric carcinoma,pericancerous and normal gastric mucosa were 75.7%,27.8% and 37.5%,respectively.The positive rates of PI3K protein in gastric cancer were significantly associated with the degree of differentiation,lymph node metastasis,depth of invasion,diffuse-type gastric cancer（P0.05）,and not associated with tumor size（P0.05）.The positive rates of AKT2 protein in gastric cancer were significantly associated with the degree of differentiation,lymph node metastasis,depth of invasion,diffuse-type gastric cancer and tumor size（P0.05）.A survival advantage was noted in the patients with PI3K/AKT2-positive tumors,independent of type,grade,or stage of the disease（P0.001）. Conclusion Over-expression of PI3K and AKT2 occurs frequently in gastric carcinoma,which may be involved in tumor development,differentiation,invasion,metastasis of gastric cancer.
出处
《实用肿瘤杂志》
CAS
北大核心
2011年第4期346-350,共5页
Journal of Practical Oncology
基金
国家自然科学基金(项目编号30872478)
