摘要
[目的]通过对黄花月见草(Oenothera glazioviana)幼苗根系铜胁迫下蛋白质组变化的研究,从蛋白质表达水平上来阐述黄花月见草根系铜胁迫响应分子机制。[方法]采用水培方法比较0、25、50和100μmol·L^(-1)铜浓度处理下黄花月见草生长状况和植株内铜元素含量,运用基于质谱的非标记相对定量技术(label-free)筛选差异表达蛋白组。[结果]黄花月见草能够耐受低浓度(50μmol·L^(-1))铜胁迫,而高浓度(100μmol·L^(-1))铜胁迫使黄花月见草生长受到抑制,根系幼小、发黄,根系中铜含量显著增加。此外,根系蛋白组检测到46个差异表达趋势蛋白(DEP),这些蛋白主要参与蛋白质加工和降解(30%)、碳水化合物与能量代谢(39%)、信号传导(22%)、生长发育(7%)和抗氧化(2%)等生物学进程。[结论]本文揭示了黄花月见草根系在铜胁迫下差异蛋白组响应表达趋势,研究结果将为探索黄花月见草铜响应分子机制提供新线索。
[Objectives]In order to explore the function of proteomics in roots of O. glazioviana seedlings,proteins of O. glazioviana roots under different copper（ Cu） concentrations were studied using proteomics technique. This work will clarify O. glazioviana roots to copper stress response molecular mechanisms. [Methods]The solution culture experiment was carried out to study the growth index,Cu contents,and based on label-free and liquid chromatography-mass spectrometry（ LC-MS） to identify proteomics,and further filter differential expression of protein（ DEP）. [Results]The results indicated that the growth of O. glazioviana was inhibited,the influence was closely related to Cu dose,roots turn yellow and little and the copper contents were increased significantly in root. In addition,the proteomics were analyzed,and 46 DEP were detected. These identified proteins were mainly involved in protein synthesis/degradation process（ 30%）,carbohydrate and energy metabolism（ 39%）,signal transduction（ 22%）,development（ 7%）and oxidoreduction（ 2%）. [Conclusions]This work gives a global insight into the characteristics of proteome of O. glazioviana roots under Cu stress,and the results could explain new clues for the study of the molecular mechanism of copper response of O. glazioviana.
出处
《南京农业大学学报》
CAS
CSCD
北大核心
2016年第5期754-762,共9页
Journal of Nanjing Agricultural University
基金
江苏省科技厅项目(BE2016812-3
BE2015680
BE2014742)
国家自然科学基金项目(41571307
31371545)
关键词
黄花月见草
铜
蛋白质组学
差异表达蛋白
Oenothera glazioviana
copper
proteomics
differential expression protein
