摘要
目的探讨β-环连蛋白抑制基因1(dapper antagonist of catenin-1,DACT1)对Ⅰ型上皮性卵巢癌迁移侵袭能力的影响及可能机制。方法免疫组织化学检测Ⅰ型人卵巢癌临床组织中DACT1的表达;采用人黏液性卵巢癌细胞株3AO构建外源性转染DACT1的稳转株,通过划痕实验、Transwell迁移及基质胶侵袭实验检测细胞运动能力;免疫荧光观察DACT1对细胞骨架重塑的影响。结果Ⅰ型卵巢癌组织中DACT1表达低于正常卵巢组织(P<0.01)。外源性转染DACT1后3AO细胞株迁移侵袭能力降低(P<0.01),且腹腔内成瘤能力有所下降,细胞骨架中特化膜结构减少。结论 DACT1对Ⅰ型卵巢癌浸润转移具有抑制效应,其在Ⅰ型卵巢癌组织中的低表达可能与其疾病发生相关。
Objective To determine the effect of dapper antagonist of catenin-1 （DACT1） on malignant invasion in type I ovarian cancer and investigate the plausible mechanism. Methods The expression of DACT1 in type I ovarian cancer tissue samples was detected by immunohistochemical assay. Human mucous ovarian cancer 3AO cells which expressed DACT1 at low endogenous level, were transfected by the lentivirus vectors carrying full-length DACT1. Wound healing, Transwell chamber assay and matrigel invasion assay were performed to measure cell motility. F-actin was detected by immunofluorescence assay for the effect of DACT1 on cytoskeletal remodeling. Results Expression of DACT1 in type I ovarian cancer was significantly lower in ovarian cancer tissues than in normal ovarian tissues （ P 〈 0.01 ）. Up-regulation of DACT1 significantly inhibited the migration and invasion capacities （P 〈 0.01 ） , intraperitoneal tumorigenesis dissemination, and specialized membrane structure in the cytoskeleton the 3AO cells. Conclusion DACT1 inhibits invasion and motility in type I ovarian cancer, and its low expression may be associated with the pathogenesis of type I epithelial ovarian cancer.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2016年第19期2158-2163,共6页
Journal of Third Military Medical University
基金
国家自然科学基金面上项目(81072120
81572562)~~
