摘要
为研究大豆(Glycine max L.)细胞壁脯氨酸富集蛋白基因(SbPRP3)在逆境胁迫中的作用,利用实时荧光定量PCR,对SbPRP3在高盐、干旱和低温处理下的表达情况进行检测。结果显示,SbPRP3在高盐处理下表达量升高,在干旱和低温处理下表达量先升高后降低。将SbPRP3构建到植物表达载体pRI101-AN上并转化烟草,获得阳性转基因烟草3株。对转基因烟草植株进行高盐、干旱和低温胁迫处理。结果表明,与对照相比,高盐和低温处理下转基因烟草脯氨酸积累量增加,而丙二醛产生量降低。但干旱处理后转基因烟草脯氨酸和丙二醛的含量与对照相比没有显著差异。由此推测SbPRP3可以提高转基因烟草的耐盐性和耐寒性。
Plant proline-rich proteins （PRPs） are putative cell wall proteins ,which are usually associated with different abiotic stresses .The expression of SbPRP3 under abiotic stress treatments in soybean was detected by real-time fluorescence quantitative PCR .The expression of SbPRP3 increased under salt treat-ment ,w hile it increased firstly and thereafter declined under drought and cold treatments .Furthermore , the ORF of SbPRP3 was cloned into the plant expression vector of pRI101-AN and then introduced into to-bacco by Agrobacterium-mediated transformation .Three positive transgenic tobacco plants were obtained . The transgenic tobacco plants were treated with salt ,drought and cold stresses .The results showed that the transgenic plants maintained higher levels of proline and lower level of malondialdehyde compared to wild-type plants under salt and cold stresses .While the levels of proline and malondialdehyde in transgenic plants had no significant differences compared to wild-type plants under drought stress .These data indica-ted that the transgenic tobacco plants constitutively expressing SbPRP3 showed an increased tolerance to salt and cold compared to wild-type plants .
作者
翟莹
张军
杨晓杰
赵艳
陈阳
ZHAI Ying ZHANG Jun YANG Xiaojie ZHAO Yan CHEN Yang(College of Life Science and Agro-Forestry, Qiqihar Univesity, Qiqihar, Heilongjiang 161006, China Heilongiiang Institute of Veterinary Science, Qiqihar, Heilongjiang 161005, China)
出处
《西北植物学报》
CAS
CSCD
北大核心
2016年第7期1331-1336,共6页
Acta Botanica Boreali-Occidentalia Sinica
基金
黑龙江省教育厅科学技术研究项目(12541889)
