摘要
该文旨在研究六味五灵片对小鼠急性乙醇肝损伤的保护作用,并初步探讨其作用机制。实验将C57BL/6小鼠随机分成7组,即空白对照组,模型组,阳性药联苯双酯组(150 mg·kg-1),六味五灵片极低、低、较高、高剂量组(0.1,0.5,4,8 g·kg-1)。采用改良的造模方法(给药4 d后给予56%乙醇,6 g·kg-1灌胃2次,12 h/次)建立小鼠急性肝损伤模型。检测小鼠血清中ALT,AST活性及肝组织中MDA,TG,TNF-α,IL-1β的含量和GSH,SOD的活性;HE和油红O染色,观察肝组织病理学变化。结果显示,与模型组相比,六味五灵片各剂量组均能显著降低血清ALT,AST的含量(P<0.05),提高GSH,SOD活性,减少肝组织MDA,TG的生成,降低肝组织中TNF-α,IL-1β的含量(P<0.05),且呈一定剂量依赖关系。同时,HE和油红O染色结果表明,六味五灵片可显著改善乙醇引起的肝细胞肿胀度,水样变性和脂滴形成等肝组织损伤。结果表明,六味五灵片对急性乙醇肝损伤具有较好的保护作用,其保护机制可能与减轻脂质过氧化,提高抗氧化酶活性,抑制氧化应激和炎症因子过表达相关。
The protective action and the relevant mechanism of Liuwei Wuling tablet on acute alcoholic hepatic injury in mice were investigated. All the C57 BL/6 mice were divided randomly into 7 groups including blank,model,bifendate（ 150 mg·kg^-1,positive control） and experimental groups consisted of extremely low dose（ 0. 1 g·kg^-1）,low（ 0. 5 g·kg^-1）,upper（ 4 g·kg^-1） and high dose（ 8 g·kg^-1） of Liuwei Wuling tablet groups. The acute liver injury model was induced by modified method that the model,positive control and experimental groups were orally administrated 56% alcohol（ 6 g·kg^-1） twice at 12 hour intervals on the fifth day afterdrugs administration. After 12 hours,the mice were sacrificed to contribute blood and liver for biochemical and histological examinations. Compared with the model,the activities of ALT and AST in serum decreased significantly in different Liuwei Wuling tablet groups. Meanwhile,in liver tissue,the levels of TG,MDA,TNF-α and IL-1β reduced obviously while the GSH and SOD activities showed markedly increase with a dose-dependent manner. Correspondingly,the microscopically pathological differences of the liver tissue observed by HE and oil red O staining indicated that the liver cell swelling,hydropic degeneration and lipid droplets formation induced by alcohol were significantly improved,which suggested the Liuwei Wuling tablet can reduce the liver injure. In conclusion,the Liuwei Wuling tablet had the protective effect on acute alcoholic hepatic injury which maybe depended on the mechanism of relieving lipid peroxidation,elevating antioxidant enzymes activity,inhibiting oxidative stress and reducing inflammation factors expression.
作者
尹萍
崔鹤蓉
章从恩
张媛
孟雅坤
贺兰芝
刘慧敏
桑秀秀
王伽伯
柏兆方
肖小河
YIN Ping CUI He-rong ZHANG Cong-en ZHANG Yuan MENG Ya-kun HE Lan-zhi LIU Hui-min SANG Xiu-xiu WANG Jia-bo BAI Zhao-fang XIAO Xiao-he(School of Pharmacy, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China School of Pharmacy, Hunan University of Traditional Chinese Medicine, Changsha 410208, China China Military Institute of Chinese Medicine, 302 Military Hospital of China, Beijing 100039, China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2016年第19期3637-3642,共6页
China Journal of Chinese Materia Medica
基金
国家自然科学基金项目(434510Z0W)
关键词
乙醇性肝损伤
六味五灵片
保肝作用
抗氧化
alcoholic liver injury
Liuwei Wuling tablet
hepatic protective effect
antioxidant
